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    acrB multidrug efflux pump RND permease AcrB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945108, updated on 3-Dec-2024

    Summary

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    Official Symbol
    acrB
    Official Full Name
    multidrug efflux pump RND permease AcrB
    Primary source
    ECOCYC:EG11704
    Locus tag
    b0462
    See related
    ASAP:ABE-0001601
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    acrE; ECK0456
    Summary
    acrB is an acrF paralog. [More information is available at EcoGene: EG11704]. AcrB is an RND-type inner-membrane associate proton-substrate antiporter. [More information is available at EcoCyc: EG11704].
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    Genomic context

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    Sequence:
    NC_000913.3 (481254..484403, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hemolysin expression-modulating protein Hha Neighboring gene Hha toxicity modulator TomB Neighboring gene multidrug efflux pump membrane fusion lipoprotein AcrA Neighboring gene DNA-binding transcriptional repressor AcrR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Vestibules are part of the substrate path in the multidrug efflux transporter AcrB of Escherichia coli. Husain F, et al. J Bacteriol, 2011 Oct. PMID 21856849, Free PMC Article
    2. Crystallographic analysis of AcrB. Pos KM, et al. FEBS Lett, 2004 Apr 30. PMID 15111118
    3. Crucial role of Asp408 in the proton translocation pathway of multidrug transporter AcrB: evidence from site-directed mutagenesis and carbodiimide labeling. Seeger MA, et al. Biochemistry, 2009 Jun 30. PMID 19425588
    4. Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport. Murakami S, et al. J Biol Chem, 2004 Jan 30. PMID 14576158
    5. AcrB multidrug efflux pump of Escherichia coli: composite substrate-binding cavity of exceptional flexibility generates its extremely wide substrate specificity. Yu EW, et al. J Bacteriol, 2003 Oct. PMID 13129936, Free PMC Article

    See all (174) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Proximal Binding Pocket Arg717 Substitutions in Escherichia coli AcrB Cause Clinically Relevant Divergencies in Resistance Profiles.
      Title: Proximal Binding Pocket Arg717 Substitutions in Escherichia coli AcrB Cause Clinically Relevant Divergencies in Resistance Profiles.
    2. In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
      Title: In situ structure of the AcrAB-TolC efflux pump at subnanometer resolution.
    3. Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
      Title: Pyridylpiperazine-based allosteric inhibitors of RND-type multidrug efflux pumps.
    4. Metabolomics Reveal Potential Natural Substrates of AcrB in Escherichia coli and Salmonella enterica Serovar Typhimurium.
      Title: Metabolomics Reveal Potential Natural Substrates of AcrB in Escherichia coli and Salmonella enterica Serovar Typhimurium.
    5. Allosteric drug transport mechanism of multidrug transporter AcrB.
      Title: Allosteric drug transport mechanism of multidrug transporter AcrB.
    6. Studying dynamics without explicit dynamics: A structure-based study of the export mechanism by AcrB.
      Title: Studying dynamics without explicit dynamics: A structure-based study of the export mechanism by AcrB.
    7. Interactions of a Bacterial RND Transporter with a Transmembrane Small Protein in a Lipid Environment.
      Title: Interactions of a Bacterial RND Transporter with a Transmembrane Small Protein in a Lipid Environment.
    8. Substrate-dependent transport mechanism in AcrB of multidrug resistant bacteria.
      Title: Substrate-dependent transport mechanism in AcrB of multidrug resistant bacteria.
    9. Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
      Title: Discovery of multidrug efflux pump inhibitors with a novel chemical scaffold.
    10. Cryo-EM analysis of a membrane protein embedded in the liposome.
      Title: Cryo-EM analysis of a membrane protein embedded in the liposome.
    Submit: New GeneRIF Correction See all GeneRIFs (66)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables alkane transmembrane transporter activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables bile acid transmembrane transporter activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables efflux transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enterobactin transmembrane transporter activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables xenobiotic transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables xenobiotic transmembrane transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in alkane transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in bile acid and bile salt transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in enterobactin transport EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in fatty acid transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in response to antibiotic IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in response to toxic substance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in response to xenobiotic stimulus IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in xenobiotic detoxification by transmembrane export across the cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in xenobiotic transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in xenobiotic transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of efflux pump complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of efflux pump complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in periplasmic side of plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    multidrug efflux pump RND permease AcrB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      481254..484403 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414995.1 multidrug efflux pump RND permease AcrB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414995.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A895P5Q3
      Conserved Domains (1) summary
      PRK15127
      Location:1 → 1049
      PRK15127; multidrug efflux RND transporter permease subunit AcrB
    Nucleotide Protein
    Heading Accession and Version

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