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    minE Z-ring positioning protein MinE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945740, updated on 3-Dec-2024

    Summary

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    Official Symbol
    minE
    Official Full Name
    Z-ring positioning protein MinE
    Primary source
    ECOCYC:EG10598
    Locus tag
    b1174
    See related
    ASAP:ABE-0003935
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1162; minB
    Summary
    Overexpression causes filamentous biofilm formation and non-motility. [More information is available at EcoGene: EG10598]. MinE is an inhibitor of MinC, which is itself an inhibitor of septum formation. [More information is available at EcoCyc: EG10598].
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    Genomic context

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    Sequence:
    NC_000913.3 (1224279..1224545, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein YmgJ Neighboring gene pseudo Neighboring gene Z-ring positioning protein MinD Neighboring gene Z-ring positioning protein MinC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Self-organization of the MinE protein ring in subcellular Min oscillations. Derr J, et al. Phys Rev E Stat Nonlin Soft Matter Phys, 2009 Jul. PMID 19658744
    2. The sequences of MinE responsible for its subcellular localization analyzed by competitive binding method in Escherichia coli. Pérez-Rodríguez MÁ, et al. Int Microbiol, 2018 Jun. PMID 30810919
    3. Phospholipid domains determine the spatial organization of the Escherichia coli cell cycle: the membrane tectonics model. Norris V. J Theor Biol, 1992 Jan 7. PMID 1573903
    4. Direct MinE-membrane interaction contributes to the proper localization of MinDE in E. coli. Hsieh CW, et al. Mol Microbiol, 2010 Jan. PMID 20025670, Free PMC Article
    5. The topological specificity factor AtMinE1 is essential for correct plastid division site placement in Arabidopsis. Maple J, et al. Plant J, 2002 Aug. PMID 12164807

    See all (125) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles.
      Title: Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles.
    2. Investigated the exchange dynamics between major and excited states of the MinE dimer in 3 forms using 15N relaxation dispersion NMR.
      Title: Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy.
    3. The amino acid in the region (32-48) is sensitive to change MinE conformation and influence its dimerization.
      Title: The sequences of MinE responsible for its subcellular localization analyzed by competitive binding method in Escherichia coli.
    4. The MinDE reaction-diffusion system can dictate the localization of membrane proteins in a spatiotemporal manner without specific molecular interactions in vitro. These proteins apparently establish a generic, nucleotide-dependent transport mode for membrane-bound diffusive molecules based on a moving diffusion barrier.
      Title: The MinDE system is a generic spatial cue for membrane protein distribution in vitro.
    5. This review summarizes the cell-free data supporting a molecular mechanism that explains the variety of patterns supported by MinD and MinE both in and out of the cell; with an emphasis on the recent appreciation for how multiple conformational states of MinE can drive oscillation by spatiotemporally regulating MinD interaction with the membrane.
      Title: Mechanistic insights of the Min oscillator via cell-free reconstitution and imaging.
    6. the dynamic association of the MTS with the beta-sheet is fine-tuned to balance MinE's need to sense MinD on the membrane with its need to diffuse in the cytoplasm, both of which are necessary for the oscillation
      Title: MinE conformational dynamics regulate membrane binding, MinD interaction, and Min oscillation.
    7. Non-linear Min protein interactions generate harmonics that signal mid-cell division in Escherichia coli.
      Title: Non-linear Min protein interactions generate harmonics that signal mid-cell division in Escherichia coli.
    8. membrane targeting sequence defines the lower limit of the concentration-dependent wavelength of Min protein patterns while restraining MinE's ability to stimulate MinD's ATPase activity.
      Title: Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence.
    9. In the process of E. coli cell division, MinC-MinD copolymers bind to membrane, interact with FtsZ, and are disassembled by MinE.
      Title: MinCD cell division proteins form alternating copolymeric cytomotive filaments.
    10. Two MinD mutant proteins, MinD(K11A) and MinD(DeltaMTS15), are unable to form polymers with MinC
      Title: The bacterial cell division regulators MinD and MinC form polymers in the presence of nucleotide.
    Submit: New GeneRIF Correction See all GeneRIFs (24)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATPase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to ATPase regulator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in division septum site selection EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in division septum site selection IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in division septum site selection IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of division septum assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Z-ring positioning protein MinE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1224279..1224545 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415692.1 Z-ring positioning protein MinE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415692.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0A734
      Conserved Domains (1) summary
      PRK00296
      Location:1 → 86
      minE; cell division topological specificity factor MinE; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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