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    gloA lactoylglutathione lyase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946161, updated on 3-Dec-2024

    Summary

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    Official Symbol
    gloA
    Official Full Name
    lactoylglutathione lyase
    Primary source
    ECOCYC:G6891
    Locus tag
    b1651
    See related
    ASAP:ABE-0005520
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1647
    Summary
    Glyoxalase I catalyzes the first of two sequential steps in the conversion of methylgloxal to D-lactate. [More information is available at EcoCyc: G6891].
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    Genomic context

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    Sequence:
    NC_000913.3 (1727837..1728244)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor NemR Neighboring gene N-ethylmaleimide reductase Neighboring gene ribonuclease T Neighboring gene ATP-dependent helicase Lhr

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. An XAS investigation of product and inhibitor complexes of Ni-containing GlxI from Escherichia coli: mechanistic implications. Davidson G, et al. Biochemistry, 2001 Apr 17. PMID 11294624
    2. Growth inhibitory properties of aromatic alpha-ketoaldehydes toward bacteria and yeast. Comparison of inhibition and glyoxalase I activity. Vander Jagt DL. J Med Chem, 1975 Nov. PMID 1100829
    3. Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences. Clugston SL, et al. Gene, 1997 Feb 20. PMID 9047352
    4. Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions. Clugston SL, et al. Biochemistry, 1998 Jun 16. PMID 9628737
    5. Structure/function relationships in nickel metallobiochemistry. Maroney MJ. Curr Opin Chem Biol, 1999 Apr. PMID 10226043

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Glyoxalase I alone among the core methylglyoxal protective systems is induced in response to methylglyoxal exposure.
      Title: Integrated stress response of Escherichia coli to methylglyoxal: transcriptional readthrough from the nemRA operon enhances protection through increased expression of glyoxalase I.
    2. We propose that cells have a cytosolic system consisting of the nemRA-gloA operon for the reduction of electrophiles, especially quinones and glyoxals, to maintain an appropriate intracellular redox balance.
      Title: Novel regulatory system nemRA-gloA for electrophile reduction in Escherichia coli K-12.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables lactoylglutathione lyase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables lactoylglutathione lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lactoylglutathione lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nickel cation binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to toxic substance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    lactoylglutathione lyase
    NP_416168.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1727837..1728244
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416168.1 lactoylglutathione lyase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416168.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3L5H119, A0A3P5DQY7
      Conserved Domains (1) summary
      TIGR00068
      Location:2 → 135
      glyox_I; lactoylglutathione lyase
    Nucleotide Protein
    Heading Accession and Version

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