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    nfo endonuclease IV [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946669, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nfo
    Official Full Name
    endonuclease IV
    Primary source
    ECOCYC:EG10651
    Locus tag
    b2159
    See related
    ASAP:ABE-0007146
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2152
    Summary
    Damaged bases in DNA occur due to alkylating agents, ionizing radiation or oxidants such as superoxide radicals, hydrogen peroxide and hydroxyl radicals. [More information is available at EcoCyc: EG10651].
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    Genomic context

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    Sequence:
    NC_000913.3 (2250840..2251697)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional regulator YeiE Neighboring gene PF03601 family inner membrane protein YeiH Neighboring gene putative sugar kinase YeiI Neighboring gene putative nucleoside transporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. High efficiency detection of bi-stranded abasic clusters in gamma-irradiated DNA by putrescine. Georgakilas AG, et al. Nucleic Acids Res, 2002 Jul 1. PMID 12087163, Free PMC Article
    2. The enzymology of apurinic/apyrimidinic endonucleases. Doetsch PW, et al. Mutat Res, 1990 Sep-Nov. PMID 1697933
    3. Endonuclease IV (nfo) mutant of Escherichia coli. Cunningham RP, et al. J Bacteriol, 1986 Dec. PMID 2430946, Free PMC Article
    4. Homogeneous Escherichia coli endonuclease IV. Characterization of an enzyme that recognizes oxidative damage in DNA. Levin JD, et al. J Biol Chem, 1988 Jun 15. PMID 2453510
    5. The multiple activities of Escherichia coli endonuclease IV and the extreme lability of 5'-terminal base-free deoxyribose 5-phosphates. Bailly V, et al. Biochem J, 1989 May 1. PMID 2471513, Free PMC Article

    See all (42) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Biochemical, mutational & crystallographic characterization reveal a 3-metal ion mechanism for damage binding and incision; DNA-free and DNA-substrate complex structures capture substrate stabilization by Arg37 and show a distorted Zn3 ligand arrangement.
      Title: DNA apurinic-apyrimidinic site binding and excision by endonuclease IV.
    2. Novel Nfo substrates alpha-2'-deoxyadenosine and alpha-thymidine play an important role in counteracting oxidative DNA base damage.
      Title: Alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease IV-type AP endonucleases.
    3. Endonuclease IV and Exonuclease III have roles in counteracting DNA damage induced by the oxidative counterpart of UVB in E.coli
      Title: Endonuclease IV and exonuclease III are involved in the repair and mutagenesis of DNA lesions induced by UVB in Escherichia coli.
    4. analysis of the three-metal-ion catalytic mechanism of the DNA repair enzyme endonuclease IV
      Title: Unraveling the three-metal-ion catalytic mechanism of the DNA repair enzyme endonuclease IV.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5'-DNA exonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables deoxyribonuclease IV (phage-T4-induced) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphoric diester hydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphoric diester hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in base-excision repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within base-excision repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IC
    Inferred by Curator
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    endonuclease IV

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2250840..2251697
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416664.1 endonuclease IV [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416664.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9Q7X6
      Conserved Domains (1) summary
      PRK01060
      Location:1 → 282
      PRK01060; endonuclease IV; Provisional
    Nucleotide Protein
    Heading Accession and Version

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