tktA transketolase 1 [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: tktA
Official Full Name: transketolase 1
Primary source: ECOCYC:EG11427
Locus tag: b2935
See related: ASAP:ABE-0009625
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2930; tkt
Summary: Mutations affect chromosomal supercoliing. [More information is available at EcoGene: EG11427]. TktA is responsible for the major transketolase activity in Escherichia coli, and TktB is responsible for the minor transketolase activity . [More information is available at EcoCyc: EG11427].
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Genomic context

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Sequence:: NC_000913.3 (3079644..3081635, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fine-tuning the activity and stability of an evolved enzyme active-site through noncanonical amino-acids.
Title: Fine-tuning the activity and stability of an evolved enzyme active-site through noncanonical amino-acids.
Molecular dynamics simulations confirmed that the mutations of TK rigidified the active-site via the correlated network.
Title: Exploiting correlated molecular-dynamics networks to counteract enzyme activity-stability trade-off.
Report the crystal structure of the S385Y/D469T/R520Q variant, and molecular docking of three substrates. This now supports our original hypothesis that directed-evolution had generated an evolutionary intermediate with divergent binding modes for the three aromatic aldehydes tested. The new active site contained two binding pockets supporting pi-pi stacking interactions, sterically separated by the D469T mutation.
Title: Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes.
In the present study, ppsA and tktA genes were overexpressed in genetically engineered Escherichia coli to enhance the availability of two precursor substrates, phosphoenolpyruvate and erythrose-4-phosphate.
Title: Improved production of tryptophan in genetically engineered Escherichia coli with TktA and PpsA overexpression.
An insertion in tktA that gave synthetic growth defects in a ppGpp0 led to the identification of tktB as a transcriptional target of ppGpp.
Title: Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB.
N-terminus verified by Edman degradation on complete protein
Title: Location of the transketolase (tkt) gene on the Escherichia coli physical map.
exponential growth-dependent expression mediated by RpoS action
Title: RpoS-mediated growth-dependent expression of the Escherichia coli tkt genes encoding transketolases isoenzymes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables manganese ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables thiamine pyrophosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables transketolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transketolase activity	IDA Inferred from Direct Assay more info	PubMed
enables transketolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in pentose-phosphate shunt	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within pentose-phosphate shunt, non-oxidative branch	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transketolase 1

YP_026188.1

EC 2.2.1.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3079644..3081635 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

YP_026188.1 transketolase 1 [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for YP_026188.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A1V2GNC3, A0A376RE42

Conserved Domains (1) summary

PRK12753
Location:1 → 663

PRK12753; transketolase; Reviewed