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    mscL large conductance mechanosensitive channel [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947787, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mscL
    Official Full Name
    large conductance mechanosensitive channel
    Primary source
    ECOCYC:EG11180
    Locus tag
    b3291
    See related
    ASAP:ABE-0010790
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3277; yhdC
    Summary
    The Escherichia coli PDB structures for MscL are homology models. otherPDB (M. tuberculosis): [More information is available at EcoGene: EG11180]. MscL is the mechanosensitive channel of large conductance that is involved in the transduction of membrane stress (mechanical or osmotic) into an electrochemical response . [More information is available at EcoCyc: EG11180].
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    Genomic context

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    Sequence:
    NC_000913.3 (3438024..3438434)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 16S rRNA m(5)C967 methyltransferase Neighboring gene NAD-binding component of Trk potassium transporters Neighboring gene alternative ribosome-rescue factor A Neighboring gene DNA-binding transcriptional activator ZntR Neighboring gene DUF1992 domain-containing protein YhdN

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dynamical properties of the MscL of Escherichia coli: a normal mode analysis. Valadié H, et al. J Mol Biol, 2003 Sep 19. PMID 12963374
    2. Channel gating: twist to open. Biggin PC, et al. Curr Biol, 2001 May 1. PMID 11369249
    3. Structural models of the MscL gating mechanism. Sukharev S, et al. Biophys J, 2001 Aug. PMID 11463635, Free PMC Article
    4. Site-directed spin-labeling analysis of reconstituted Mscl in the closed state. Perozo E, et al. J Gen Physiol, 2001 Aug. PMID 11479346, Free PMC Article
    5. A mechanosensitive channel protein and its gene in E. coli. Blount P, et al. Gravit Space Biol Bull, 1997 Jun. PMID 11540118

    See all (166) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
      Title: A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
    2. Interaction between mechanosensitive channels embedded in lipid membrane.
      Title: Interaction between mechanosensitive channels embedded in lipid membrane.
    3. The study described a novel class of MscL mutations that were correlated with the thermodynamic stability of the MscL structure.
      Title: Temperature-sensitive mutants of MscL mechanosensitive channel.
    4. Collectively, the findings indicate that MscL-dependent protein excretion is positively regulated in response to both osmotic stress and arfA-mediated translational stress.
      Title: Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins.
    5. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices.
      Title: Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.
    6. the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane
      Title: The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels.
    7. More importantly, the FE model indicates that to fully operate an MscL nanovalve by pulling directly on the N-terminus and bottom of TM1, gain-of-function (GOF) mutants (e.g., G22N MscL) would have to be employed rather than the WT MscL channel.
      Title: Pulling MscL open via N-terminal and TM1 helices: A computational study towards engineering an MscL nanovalve.
    8. Data suggest that dihydrostreptomycin binds to a specific site on mechanosensitive channel of large conductance (MscL) and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell.
      Title: Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore.
    9. comprehensive screen has identified many residues that are critical for channel function as well as highlighted MscL domains and residues that undergo the most drastic environmental changes upon gating.
      Title: Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations.
    10. Observe directly that the MscL channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter.
      Title: Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel.
    Submit: New GeneRIF Correction See all GeneRIFs (25)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables mechanosensitive monoatomic ion channel activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables mechanosensitive monoatomic ion channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables mechanosensitive monoatomic ion channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mechanosensitive monoatomic ion channel activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mechanosensitive monoatomic ion channel activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within intracellular water homeostasis EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within monoatomic ion transmembrane transport EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within monoatomic ion transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in monoatomic ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within monoatomic ion transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane HDA PubMed 
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    large conductance mechanosensitive channel

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3438024..3438434
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417749.1 large conductance mechanosensitive channel [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417749.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FJL6
      Conserved Domains (1) summary
      PRK00567
      Location:3 → 136
      mscL; large-conductance mechanosensitive channel protein MscL
    Nucleotide Protein
    Heading Accession and Version

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