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    rplB 50S ribosomal subunit protein L2 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947820, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rplB
    Official Full Name
    50S ribosomal subunit protein L2
    Primary source
    ECOCYC:EG10865
    Locus tag
    b3317
    See related
    ASAP:ABE-0010854
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3304
    Summary
    The L2 protein is a component of the 50S subunit of the ribosome and is part of the peptidyltransferase center. [More information is available at EcoCyc: EG10865].
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    Genomic context

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    Sequence:
    NC_000913.3 (3450543..3451364, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L22 Neighboring gene 30S ribosomal subunit protein S19 Neighboring gene 50S ribosomal subunit protein L23 Neighboring gene 50S ribosomal subunit protein L4 Neighboring gene 50S ribosomal subunit protein L3

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The properties of the complex between ribosomal protein L2 and tRNA. Remme J, et al. FEBS Lett, 1985 Oct 14. PMID 2412896
    2. Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic-binding sequence for ribosomal protein L2. Kitahara K, et al. Nucleic Acids Res, 2007. PMID 17553838, Free PMC Article
    3. A ribosomal protein that is immunologically conserved in archaebacteria, eubacteria and eukaryotes. Schmid G, et al. FEBS Lett, 1984 Nov 19. PMID 6209167
    4. The Si-tag for immobilizing proteins on a silica surface. Taniguchi K, et al. Biotechnol Bioeng, 2007 Apr 15. PMID 17013933
    5. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer. Diedrich G, et al. EMBO J, 2000 Oct 2. PMID 11013226, Free PMC Article

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A truncated form of L2 or the mature L2 physically interacts with the N-terminal region of DnaA to inhibit initiation from oriC by apparently interfering with DnaA oligomer formation.
      Title: Two forms of ribosomal protein L2 of Escherichia coli that inhibit DnaA in DNA replication.
    2. The direct interaction between RNAPalpha and RPL2 was confirmed both in vivo and in vitro by performing coimmunoprecipitation and bacterial two-hybrid experiments.
      Title: The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Structure of the Escherichia coli S10 ribosomal protein operon.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cytoplasmic translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ribosomal large subunit assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of DnaA-L2 complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of cytosolic large ribosomal subunit IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic large ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of large ribosomal subunit IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    50S ribosomal subunit protein L2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3450543..3451364 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417776.1 50S ribosomal subunit protein L2 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417776.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A090B4C0, A0A7A7A9X5
      Conserved Domains (1) summary
      PRK09374
      Location:1 → 273
      rplB; 50S ribosomal protein L2; Validated
    Nucleotide Protein
    Heading Accession and Version

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