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    cspA cold shock protein CspA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948070, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cspA
    Official Full Name
    cold shock protein CspA
    Primary source
    ECOCYC:EG10166
    Locus tag
    b3556
    See related
    ASAP:ABE-0011616
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3543
    Summary
    The cspABEG deletion BX04 is cold-sensitive and forms filaments at 15C (Xia, 2001). [More information is available at EcoGene: EG10166]. CspA is an RNA chaperone that prevents secondary structure formation in RNA at low temperatures . [More information is available at EcoCyc: EG10166].
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    Genomic context

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    Sequence:
    NC_000913.3 (3720049..3720261)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF3053 domain-containing protein YiaF Neighboring gene putative DNA-binding transcriptional regulator YiaG Neighboring gene protein YsaE Neighboring gene small toxic polypeptide Neighboring gene pseudo Neighboring gene insertion element IS150 protein InsA Neighboring gene IS150 family conserved protein InsB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Transcription of cspA, the gene for the major cold-shock protein of Escherichia coli, is negatively regulated at 37 degrees C by the 5'-untranslated region of its mRNA. Fang L, et al. FEMS Microbiol Lett, 1999 Jul 1. PMID 10418129
    2. Deletion analysis of cspA of Escherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction. Mitta M, et al. Mol Microbiol, 1997 Oct. PMID 9383157
    3. Role of Escherichia coli cspA promoter sequences and adaptation of translational apparatus in the cold shock response. Goldenberg D, et al. Mol Gen Genet, 1997 Oct. PMID 9393453
    4. Stability and folding properties of a model beta-sheet protein, Escherichia coli CspA. Reid KL, et al. Protein Sci, 1998 Feb. PMID 9521124, Free PMC Article
    5. Extended -10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription. Phadtare S, et al. J Bacteriol, 2005 Sep. PMID 16159795, Free PMC Article

    See all (84) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A switch from alpha-helical to beta-strand conformation during co-translational protein folding.
      Title: A switch from α-helical to β-strand conformation during co-translational protein folding.
    2. A Revised Understanding of Clostridioides difficile Spore Germination.
      Title: A Revised Understanding of Clostridioides difficile Spore Germination.
    3. Study showed improved drought tolerance in wheat plants overexpressing a synthetic bacterial cold shock protein gene SeCspA.
      Title: Improved drought tolerance in wheat plants overexpressing a synthetic bacterial cold shock protein gene SeCspA.
    4. the RNA-melting activity of CspA upon binding to different RNA hairpins was determined by NMR spectroscopy
      Title: RNA binding and chaperone activity of the E. coli cold-shock protein CspA.
    5. Overexpression of both 5'-UTR and truncated CspA mutant proteins caused the LACE (low temperature-dependent antibiotic effect of truncated cspA expression) phenotype, and polysomes appeared to be stalled at stop codons.
      Title: Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs.
    6. Data propose that Hfq may play a facilitating role in the metabolism of cspA mRNA.
      Title: Interactions of the RNA-binding protein Hfq with cspA mRNA, encoding the major cold shock protein.
    7. N-terminus verified by Edman degradation on mature peptide
      Title: Major cold shock protein of Escherichia coli.
    8. Data suggest a model for the regulation of expression of CspA that rationalizes the roles of the secondary structure and RNase E.
      Title: Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA.
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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleic acid binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription antitermination factor activity, RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within negative regulation of termination of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of gene expression IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in transcription antitermination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cold shock protein CspA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3720049..3720261
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418012.1 cold shock protein CspA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418012.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FDV4
      Conserved Domains (1) summary
      PRK10354
      Location:1 → 70
      PRK10354; RNA chaperone/antiterminator CspA
    Nucleotide Protein
    Heading Accession and Version

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