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    pflA pyruvate formate-lyase activating enzyme [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945517, updated on 17-Dec-2024

    Summary

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    Official Symbol
    pflA
    Official Full Name
    pyruvate formate-lyase activating enzyme
    Primary source
    ECOCYC:EG10028
    Locus tag
    b0902
    See related
    ASAP:ABE-0003068
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    act; ECK0893
    Summary
    Radical SAM superfamily. [More information is available at EcoGene: EG10028].
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    Genomic context

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    Sequence:
    NC_000913.3 (950340..951080, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator YcaN Neighboring gene putative oxidoreductase YcaK Neighboring gene pyruvate formate-lyase Neighboring gene formate channel FocA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Specialized transduction of the biotin region of Escherichia coli by phage T1. Drexler H. Mol Gen Genet, 1977 Mar 28. PMID 325388
    2. Reconstitution and characterization of the polynuclear iron-sulfur cluster in pyruvate formate-lyase-activating enzyme. Molecular properties of the holoenzyme form. Külzer R, et al. J Biol Chem, 1998 Feb 27. PMID 9478932
    3. Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme. Shisler KA, et al. J Am Chem Soc, 2017 Aug 30. PMID 28768413, Free PMC Article
    4. Pyruvate formate-lyase (inactive form) and pyruvate formate-lyase activating enzyme of Escherichia coli: isolation and structural properties. Conradt H, et al. Arch Biochem Biophys, 1984 Jan. PMID 6364987
    5. The iron-sulfur cluster of pyruvate formate-lyase activating enzyme in whole cells: cluster interconversion and a valence-localized [4Fe-4S]2+ state. Yang J, et al. Biochemistry, 2009 Oct 6. PMID 19711960, Free PMC Article

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. N-terminus verified by Edman degradation on mature peptide
      Title: Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables [formate-C-acetyltransferase]-activating enzyme activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables [formate-C-acetyltransferase]-activating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables potassium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in glucose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein maturation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    pyruvate formate-lyase activating enzyme
    NP_415422.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      950340..951080 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415422.1 pyruvate formate-lyase activating enzyme [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415422.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FM59
      Conserved Domains (1) summary
      PRK11145
      Location:1 → 246
      pflA; pyruvate formate lyase 1-activating protein
    Nucleotide Protein
    Heading Accession and Version

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