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    metK methionine adenosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945389, updated on 3-Dec-2024

    Summary

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    Official Symbol
    metK
    Official Full Name
    methionine adenosyltransferase
    Primary source
    ECOCYC:EG10589
    Locus tag
    b2942
    See related
    ASAP:ABE-0009650
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2937; metX
    Summary
    The adjacent ORF yqgD has been removed from EcoGene because it is very unlikely to be a gene; the limited conservation with Salmonella is apparently due to a pseudotranslation of the conserved metK CRP site, promoter and the metK 5' UTR. [More information is available at EcoGene: EG10589]. Methionine adenosyltransferase catalyzes the formation of the sulfonium compound S-adenosylmethionine. [More information is available at EcoCyc: EG10589].
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    Genomic context

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    Sequence:
    NC_000913.3 (3086706..3087860)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YqgG Neighboring gene protein YqgC Neighboring gene DUF2684 domain-containing protein YqgD Neighboring gene galactose:H(+) symporter Neighboring gene protein YggI

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Energetics of S-adenosylmethionine synthetase catalysis. McQueney MS, et al. Biochemistry, 2000 Apr 18. PMID 10757994
    2. Expression of rat liver S-adenosylmethionine synthetase in Escherichia coli results in two active oligomeric forms. Alvarez L, et al. Biochem J, 1994 Jul 15. PMID 8043003, Free PMC Article
    3. Isozymes of S-adenosylmethionine synthetase are encoded by tandemly duplicated genes in Escherichia coli. Satishchandran C, et al. Mol Microbiol, 1993 Aug. PMID 8231813
    4. Coordination of vanadyl(IV) cation in complexes of S-adenosylmethionine synthetase: multifrequency electron spin echo envelope modulation study. Zhang C, et al. Biochemistry, 1993 Sep 21. PMID 8396974
    5. Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met. Komoto J, et al. Biochemistry, 2004 Feb 24. PMID 14967023

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. analysis of key features that define the GroE dependence on MetK proteins
      Title: Conversion of a chaperonin GroEL-independent protein into an obligate substrate.
    2. The metK deficient Escherichia coli mutant uses externally provided S-adenosylmethionine as methyl donor to produce most but not all of its methionine, by methylation of homocysteine catalysed by MmuM.
      Title: Cell division, one-carbon metabolism and methionine synthesis in a metK-deficient Escherichia coli mutant, and a role for MmuM.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: The cadmium-stress stimulon of Escherichia coli K-12.
    4. Data suggest that a deficiency in S-adenosylmethionine synthetase/S-adenosylmethionine stops cell division at a specific point in the septation process.
      Title: A deficiency in S-adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methionine adenosyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methionine adenosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methionine adenosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables potassium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables potassium ion binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in S-adenosylmethionine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in S-adenosylmethionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within S-adenosylmethionine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in S-adenosylmethionine cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in one-carbon metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    methionine adenosyltransferase
    NP_417417.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3086706..3087860
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417417.1 methionine adenosyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417417.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A485JD64
      Conserved Domains (1) summary
      PRK05250
      Location:3 → 383
      PRK05250; S-adenosylmethionine synthetase; Validated
    Nucleotide Protein
    Heading Accession and Version

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