greA transcription elongation factor GreA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: greA
Official Full Name: transcription elongation factor GreA
Primary source: ECOCYC:EG10415
Locus tag: b3181
See related: ASAP:ABE-0010454
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3170
Summary: HT_Cmplx24_Cyt: [More information is available at EcoGene: EG10415]. GreA stimulates the mRNA cleavage activity of RNA polymerase , which acts to release a polymerase complex that has stalled or incorporated an incorrect nucleotide . [More information is available at EcoCyc: EG10415].
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Genomic context

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Sequence:: NC_000913.3 (3328239..3328715, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.
Title: Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.
Mutational analysis of Escherichia coli GreA protein reveals new functional activity independent of antipause and lethal when overexpressed.
Title: Mutational analysis of Escherichia coli GreA protein reveals new functional activity independent of antipause and lethal when overexpressed.
greA expression is autoregulated by GraL level.
Title: Autoregulation of greA Expression Relies on GraL Rather than on greA Promoter Region.
overexpression of GreB in DeltagreA cells reduced the misincorporation errors to wild-type levels, demonstrating that GreB at high concentration could substitute for GreA in RNA proofreading activity in vivo.
Title: A Cre Transcription Fidelity Reporter Identifies GreA as a Major RNA Proofreading Factor in Escherichia coli.
GreA mutations increase the efficiency of RecA-mediated recombination.
Title: Recombination phenotypes of Escherichia coli greA mutants.
The greA leader region contains two strong promoters and an unusual terminator that gives rise to two clusters of small RNA chains.
Title: Imprecise transcription termination within Escherichia coli greA leader gives rise to an array of short transcripts, GraL.
Data show that GreA deficiency enhanced RNAP trapping at most of the newly identified stall sites.
Title: Prevalence of RNA polymerase stalling at Escherichia coli promoters after open complex formation.
transcript cleavage during early stages of initiation is thus the main in vivo function of GreA.
Title: Analysis of promoter targets for Escherichia coli transcription elongation factor GreA in vivo and in vitro.
N-terminus verified by Edman degradation on complete protein
Title: GreA protein: a transcription elongation factor from Escherichia coli.
GreA effects on rrnB P1 open complex conformation reveal a new feature of GreA distinct from its general function in elongation.
Title: Antagonistic regulation of Escherichia coli ribosomal RNA rrnB P1 promoter activity by GreA and DksA.

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase binding	IEA Inferred from Electronic Annotation more info
enables bacterial-type RNA polymerase holo enzyme binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription elongation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within DNA-templated transcription elongation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription elongation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription elongation factor GreA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3328239..3328715 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417648.4 transcription elongation factor GreA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417648.4

Status: PROVISIONAL

UniProtKB/TrEMBL

C3SSI7

Conserved Domains (1) summary

PRK00226
Location:1 → 157

greA; transcription elongation factor GreA; Reviewed