U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    dcuS sensor histidine kinase DcuS [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948639, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    dcuS
    Official Full Name
    sensor histidine kinase DcuS
    Primary source
    ECOCYC:G7827
    Locus tag
    b4125
    See related
    ASAP:ABE-0013509
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4118; yjdH
    Summary
    dcuSR operon is repressed by nitrate via the NarXL system. [More information is available at EcoGene: EG12465]. DcuS acts as a C4-dicarboxylate-sensing histidine kinase . [More information is available at EcoCyc: G7827].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4350031..4351662, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene fumarase B Neighboring gene anaerobic C4-dicarboxylate transporter DcuB Neighboring gene DNA-binding transcriptional activator DcuR Neighboring gene PF06902 family protein YjdI Neighboring gene putative N-acetyltransferase YjdJ

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. The nature of the stimulus and of the fumarate binding site of the fumarate sensor DcuS of Escherichia coli. Kneuper H, et al. J Biol Chem, 2005 May 27. PMID 15781452
    2. Properties of transmembrane helix TM1 of the DcuS sensor kinase of Escherichia coli, the stator for TM2 piston signaling. Stopp M, et al. Biol Chem, 2021 Sep 27. PMID 34355547
    3. Simultaneous measurement of protein one-bond residual dipolar couplings without increased resonance overlap. Vijayan V, et al. J Magn Reson, 2005 Jun. PMID 15862241
    4. Citrate sensing by the C4-dicarboxylate/citrate sensor kinase DcuS of Escherichia coli: binding site and conversion of DcuS to a C4-dicarboxylate- or citrate-specific sensor. Krämer J, et al. J Bacteriol, 2007 Jun. PMID 17416661, Free PMC Article
    5. The NMR structure of the sensory domain of the membranous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli. Pappalardo L, et al. J Biol Chem, 2003 Oct 3. PMID 12907689

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Properties of transmembrane helix TM1 of the DcuS sensor kinase of Escherichia coli, the stator for TM2 piston signaling.
      Title: Properties of transmembrane helix TM1 of the DcuS sensor kinase of Escherichia coli, the stator for TM2 piston signaling.
    2. Regulation of Aerobic Succinate Transporter dctA of E. coli by cAMP-CRP, DcuS-DcuR, and EIIAGlc: Succinate as a Carbon Substrate and Signaling Molecule.
      Title: Regulation of Aerobic Succinate Transporter dctA of E. coli by cAMP-CRP, DcuS-DcuR, and EIIAGlc: Succinate as a Carbon Substrate and Signaling Molecule.
    3. Tight Complex Formation of the Fumarate Sensing DcuS-DcuR Two-Component System at the Membrane and Target Promoter Search by Free DcuR Diffusion.
      Title: Tight Complex Formation of the Fumarate Sensing DcuS-DcuR Two-Component System at the Membrane and Target Promoter Search by Free DcuR Diffusion.
    4. The relationship of absolute contents of DcuS, DcuB, and DctA to the functional state of DcuS revealed their contribution to the control of DcuS-DcuR in vivo, which was not accessible by other approaches, leading to a revision of previous models.
      Title: Cellular Concentrations of the Transporters DctA and DcuB and the Sensor DcuS of Escherichia coli and the Contributions of Free and Complexed DcuS to Transcriptional Regulation by DcuR.
    5. DcuS is required for location of DctA and DcuR at the poles and formation of tripartite DctA/DcuS/DcuR sensor/regulator complexes.
      Title: Polar localization of a tripartite complex of the two-component system DcuS/DcuR and the transporter DctA in Escherichia coli depends on the sensor kinase DcuS.
    6. C4-dicarboxylate sensing by DctA/DcuS resides in DcuS, and that DctA serves as an activity switch.
      Title: Differentiation of DctA and DcuS function in the DctA/DcuS sensor complex of Escherichia coli: function of DctA as an activity switch and of DcuS as the C4-dicarboxylate sensor.
    7. Reorganizing the DcuS homodimer and its interaction with DctA activate the kinase.
      Title: The cytoplasmic PASC domain of the sensor kinase DcuS of Escherichia coli: role in signal transduction, dimer formation, and DctA interaction.
    8. It is concluded that DctA forms a functional unit or sensor complex with DcuS through specific interaction sites.
      Title: Interaction of the Escherichia coli transporter DctA with the sensor kinase DcuS: presence of functional DctA/DcuS sensor units.
    9. DcuS/DcuR modulating citA and citB expression.
      Title: CitA/CitB two-component system regulating citrate fermentation in Escherichia coli and its relation to the DcuS/DcuR system in vivo.
    10. The FRET and cross-linking data demonstrate the presence in living cells, in bacterial membranes, and in proteoliposomes of full-length DcuS protein in an oligomeric state, including a tetramer.
      Title: Oligomeric sensor kinase DcuS in the membrane of Escherichia coli and in proteoliposomes: chemical cross-linking and FRET spectroscopy.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphorelay sensor kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphorelay sensor kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay sensor kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein histidine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein histidine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within phosphorelay signal transduction system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within signal transduction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    part_of protein histidine kinase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    sensor histidine kinase DcuS
    NP_418549.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4350031..4351662 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418549.1 sensor histidine kinase DcuS [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418549.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7L7EBX4
      Conserved Domains (1) summary
      PRK11086
      Location:2 → 543
      PRK11086; sensory histidine kinase DcuS; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.