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    ftsI peptidoglycan DD-transpeptidase FtsI [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944799, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ftsI
    Official Full Name
    peptidoglycan DD-transpeptidase FtsI
    Primary source
    ECOCYC:EG10341
    Locus tag
    b0084
    See related
    ASAP:ABE-0000309
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0085; pbpB; sep
    Summary
    FtsI is recruited by its cognate SEDS protein FtsW. [More information is available at EcoGene: EG10341]. FtsI (penicillin-binding protein 3, PBP3) is an essential cell division protein which is present at low abundance of about 100 molecules per cell . [More information is available at EcoCyc: EG10341].
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    Genomic context

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    Sequence:
    NC_000913.3 (91413..93179)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor MraZ Neighboring gene 16S rRNA m(4)C1402 methyltransferase Neighboring gene cell division protein FtsL Neighboring gene ncRNA Neighboring gene UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Neighboring gene D-alanyl-D-alanine-adding enzyme Neighboring gene phospho-N-acetylmuramoyl-pentapeptide-transferase Neighboring gene UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase Neighboring gene peptidoglycan glycosyltransferase FtsW

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition. Aedo SJ, et al. BMC Microbiol, 2019 Jun 24. PMID 31234796, Free PMC Article
    2. Expression of the Escherichia coli cell division gene sep cloned in a lambda Charon phage. Irwin CA, et al. Science, 1979 Oct 12. PMID 158216
    3. Identification of a new mutation in Escherichia coli that suppresses a pbpB (Ts) phenotype in the presence of penicillin-binding protein 1B. García del Portillo F, et al. FEMS Microbiol Lett, 1991 Nov 1. PMID 1769557
    4. Membrane topology of penicillin-binding protein 3 of Escherichia coli. Bowler LD, et al. Mol Microbiol, 1989 Sep. PMID 2677607
    5. An amino acid substitution in penicillin-binding protein 3 creates pointed polar caps in Escherichia coli. Taschner PE, et al. J Bacteriol, 1988 Oct. PMID 3049550, Free PMC Article

    See all (95) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data suggest that piperacillin and more generally PBP3 inhibition during stationary phase produces a phenotypic state characterized by large abundance of essential growth materials that render cells more uniformly susceptible to the bactericidal activities of beta-lactams and fluoroquinolones.
      Title: Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition.
    2. A beta-lactam MIC of 8 mug/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.
      Title: Unusual Escherichia coli PBP 3 Insertion Sequence Identified from a Collection of Carbapenem-Resistant Enterobacteriaceae Tested In Vitro with a Combination of Ceftazidime-, Ceftaroline-, or Aztreonam-Avibactam.
    3. In this work the authors show direct interactions between FtsW and PBP3 in vivo and in vitro by FRET (Forster resonance energy transfer) and co-immunoprecipitation experiments.
      Title: The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli.
    4. Data show that PBP3 interact with MtgA and help MtgA's role in peptidoglycan assembly during the cell cycle.
      Title: The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN.
    5. FtsI is associated in a hierarchical set of protein complexes and its assembly can occur independently of 'upstream' proteins.
      Title: Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly.
    6. Alanine-scanning mutagenesis of the Transmembrane helix identified several residues important for septal localization.
      Title: The transmembrane helix of the Escherichia coli division protein FtsI localizes to the septal ring.
    7. direct interaction between a class B PBP (PBP3) and a class A PBP (PBP1B) in vitro and in vivo, indicating that different murein synthases might act in concert to enlarge the murein sacculus during cell division
      Title: Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carboxypeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables penicillin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables penicillin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables penicillin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidoglycan glycosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables peptidoglycan glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidoglycan glycosyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables serine-type D-Ala-D-Ala carboxypeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in FtsZ-dependent cytokinesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in FtsZ-dependent cytokinesis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell division IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cell division NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell wall organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in division septum assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in division septum assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in peptidoglycan biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within peptidoglycan biosynthetic process RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cell shape IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to xenobiotic stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell division site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell division site NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of divisome complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    peptidoglycan DD-transpeptidase FtsI

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      91413..93179
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414626.1 peptidoglycan DD-transpeptidase FtsI [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414626.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3G1CH64
      Conserved Domains (1) summary
      PRK15105
      Location:2 → 579
      PRK15105; peptidoglycan synthase FtsI; Provisional
    Nucleotide Protein
    Heading Accession and Version

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