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    araC DNA-binding transcriptional dual regulator AraC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944780, updated on 17-Dec-2024

    Summary

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    Official Symbol
    araC
    Official Full Name
    DNA-binding transcriptional dual regulator AraC
    Primary source
    ECOCYC:EG10054
    Locus tag
    b0064
    See related
    ASAP:ABE-0000236
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0065
    Summary
    AraC activates transcrption for araE and the araBAD and araFGH operons; AraC transcriptional activator family prototype. [More information is available at EcoGene: EG10054]. The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. [More information is available at EcoCyc: EG10054].
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    Genomic context

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    Sequence:
    NC_000913.3 (70387..71265)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene L-arabinose isomerase Neighboring gene ribulokinase Neighboring gene DedA family protein YabI Neighboring gene thiamine ABC transporter ATP binding subunit Neighboring gene thiamine ABC transporter membrane subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Transcription start site and induction kinetics of the araC regulatory gene in Escherichia coli K-12. Stoner CM, et al. J Mol Biol, 1983 Nov 15. PMID 6196488
    2. The interaction of L-arabinose and D-fucose with AraC protein. Wilcox G. J Biol Chem, 1974 Nov 10. PMID 4608387
    3. Direction of transcription of the regulatory gene araC in Escherichia coli B-r. Wilcox G, et al. Proc Natl Acad Sci U S A, 1974 Sep. PMID 4610582, Free PMC Article
    4. Isolation of araC protein by affinity chromatography. Wilcox G, et al. Methods Enzymol, 1974. PMID 4615240
    5. Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r. Irr J, et al. Genetics, 1970 May. PMID 4920896, Free PMC Article

    See all (119) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Atomic force microscopy measurements and model of DNA bending caused by binding of AraC protein.
      Title: Atomic force microscopy measurements and model of DNA bending caused by binding of AraC protein.
    2. Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
      Title: Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
    3. Engineering AraC to make it responsive to light instead of arabinose.
      Title: Engineering AraC to make it responsive to light instead of arabinose.
    4. Arabinose binding to the AraC dimerization domain changes the properties of the AraC inter-domain linker which connects the dimerization domain to the DNA binding domain via interactions that do not depend on the DNA binding domain.
      Title: A genetic and physical study of the interdomain linker of E. Coli AraC protein--a trans-subunit communication pathway.
    5. Data [including data from studies of mutant AraC (P8G, P8A)] suggest that, in AraC conformation, interaction of the N-terminal arm with something other than the front lip is the primary determinant of the inducing versus repressing state of AraC.
      Title: Computational and experimental investigation of constitutive behavior in AraC.
    6. Interaction between domains of AraC in presence of arabinose is reported. Dissociation of arabinose-bound AraC from half-site DNA is faster than free DNA binding domain;affinity of dimeriz'n domains for arabinose is increased when half-site DNA is bound.
      Title: A new and unexpected domain-domain interaction in the AraC protein.
    7. Mutation in the interdomain affect the dimerization domain-DNA-binding domain interactions in AraC.
      Title: Active role of the interdomain linker of AraC.
    8. The majority of the constitutive mutations lie within the same residues of the N-terminal regulatory arm of AraC. Two, however, were found in the core of the dimerization domain.
      Title: Constitutive mutations in the Escherichia coli AraC protein.
    9. N-terminus verified by Edman degradation on mature peptide
      Title: The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence.
    10. The allosteric mechanism by which the gene expression regulatory protein AraC regulates its DNA-binding activity is shown to be portable.
      Title: A portable allosteric mechanism.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription repressor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transcription cis-regulatory region binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in arabinose catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within arabinose catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of primary metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-DNA complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator AraC

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      70387..71265
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414606.1 DNA-binding transcriptional dual regulator AraC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414606.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N0IPE6
      Conserved Domains (1) summary
      PRK10572
      Location:1 → 285
      PRK10572; arabinose operon transcriptional regulator AraC
    Nucleotide Protein
    Heading Accession and Version

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