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    nfsB NAD(P)H-dependent nitroreductase NfsB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945778, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nfsB
    Official Full Name
    NAD(P)H-dependent nitroreductase NfsB
    Primary source
    ECOCYC:EG50005
    Locus tag
    b0578
    See related
    ASAP:ABE-0001981
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dprA; ECK0570; nfnB; ntr
    Summary
    nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG20151]. Aspartate 37 has been implicated in binding the NADH cofactor. [More information is available at EcoCyc: EG50005].
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    Genomic context

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    Sequence:
    NC_000913.3 (604771..605424, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene phenylalanine:H(+) symporter PheP Neighboring gene miniconductance mechanosensitive channel YbdG Neighboring gene PF04237 family protein YbdF Neighboring gene DUF1158 domain-containing protein YbdJ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)--I. Purification and properties of a nitroreductase enzyme from Escherichia coli--a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT). Anlezark GM, et al. Biochem Pharmacol, 1992 Dec 15. PMID 1472094
    2. Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954. Grove JI, et al. Cancer Res, 2003 Sep 1. PMID 14500391
    3. The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution. Lovering AL, et al. J Mol Biol, 2001 May 25. PMID 11491290
    4. Sensitization of colorectal and pancreatic cancer cell lines to the prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954) by retroviral transduction and expression of the E. coli nitroreductase gene. Green NK, et al. Cancer Gene Ther, 1997 Jul-Aug. PMID 9253508
    5. Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri. Zenno S, et al. J Biochem, 1996 Oct. PMID 8947835

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
      Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
    2. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
      Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
    3. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
      Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.
    4. investigation of flavin-independent Fenton reaction system; NfnB and Fpr were purified and showed catalytic activities of ferric reduction; characterization of these enzymes and their involvement in the Fenton reaction and ferric reduction are presented
      Title: Escherichia coli ferredoxin-NADP+ reductase and oxygen-insensitive nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    6. Data describe the interaction of Escherichia coli nitroreductase with the antibiotic nitrofurazone.
      Title: Structural and mechanistic studies of Escherichia coli nitroreductase with the antibiotic nitrofurazone. Reversed binding orientations in different redox states of the enzyme.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 6,7-dihydropteridine reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 6,7-dihydropteridine reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables FMN binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NAD(P)H dehydrogenase (quinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables oxidoreductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in 2,4,6-trinitrotoluene catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within 2,4,6-trinitrotoluene catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    NAD(P)H-dependent nitroreductase NfsB
    NP_415110.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      604771..605424 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415110.1 NAD(P)H-dependent nitroreductase NfsB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415110.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      F2X7N5
      Conserved Domains (1) summary
      PRK11053
      Location:1 → 217
      PRK11053; oxygen-insensitive NAD(P)H nitroreductase
    Nucleotide Protein
    Heading Accession and Version

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