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    nei endonuclease VIII [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945320, updated on 17-Dec-2024

    Summary

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    Official Symbol
    nei
    Official Full Name
    endonuclease VIII
    Primary source
    ECOCYC:G6383
    Locus tag
    b0714
    See related
    ASAP:ABE-0002433
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0703
    Summary
    Damaged bases in DNA occur due to alkylating agents, ionizing radiation or oxidants such as superoxide radicals, hydrogen peroxide and hydroxyl radicals. [More information is available at EcoCyc: G6383].
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    Genomic context

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    Sequence:
    NC_000913.3 (745935..746726)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 5-oxoprolinase component B Neighboring gene 5-oxoprolinase component C Neighboring gene 5-oxoprolinase component A Neighboring gene putative regulator Neighboring gene putative fimbrial protein YbgO Neighboring gene putative fimbrial chaperone YbgP Neighboring gene putative fimbrial usher protein YbgQ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Conformational dynamics of the interaction of Escherichia coli endonuclease VIII with DNA substrates. Kuznetsov NA, et al. DNA Repair (Amst), 2012 Nov 1. PMID 23000248
    2. Isolation and characterization of endonuclease VIII from Escherichia coli. Melamede RJ, et al. Biochemistry, 1994 Feb 8. PMID 8110759
    3. Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants. Jiang D, et al. J Bacteriol, 1997 Jun. PMID 9171429, Free PMC Article
    4. Characterization of Escherichia coli endonuclease VIII. Jiang D, et al. J Biol Chem, 1997 Dec 19. PMID 9405426
    5. A novel role for Escherichia coli endonuclease VIII in prevention of spontaneous G-->T transversions. Blaisdell JO, et al. J Bacteriol, 1999 Oct. PMID 10515930, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases.
      Title: Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases.
    2. A study of conformational changes in the Nei molecule when the enzyme binds normal DNA, damaged but uncleavable DNA, and several cleavable damaged DNA substrates.
      Title: Conformational dynamics of the interaction of Escherichia coli endonuclease VIII with DNA substrates.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.
    4. Endonuclease VIII does not protect E.coli against possible mutations attributable to formamidopyrimidine lesions.
      Title: Excision of formamidopyrimidine lesions by endonucleases III and VIII is not a major DNA repair pathway in Escherichia coli.
    5. The structure of the uncomplexed Nei shows a significant conformational change compared with the Nei structure in its complex with DNA, reflecting a approximately 50 degrees rotation of the two main domains of the enzyme.
      Title: Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility.
    6. crystal structure
      Title: Crystallization and preliminary crystallographic analysis of endonuclease VIII in its uncomplexed form.
    7. Nei base excision repair enzyme is critical for the recognition and repair of the spiroiminodihydantoin lesion in bacterial cell lines
      Title: Nei deficient Escherichia coli are sensitive to chromate and accumulate the oxidized guanine lesion spiroiminodihydantoin.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables damaged DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables damaged DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within base-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in base-excision repair IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    endonuclease VIII

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      745935..746726
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415242.1 endonuclease VIII [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415242.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NQB2
      Conserved Domains (1) summary
      PRK10445
      Location:1 → 263
      PRK10445; endonuclease VIII; Provisional
    Nucleotide Protein
    Heading Accession and Version

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