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    mukF chromosome partitioning protein MukF [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945548, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mukF
    Official Full Name
    chromosome partitioning protein MukF
    Primary source
    ECOCYC:EG12165
    Locus tag
    b0922
    See related
    ASAP:ABE-0003134
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0913; kicB
    Summary
    Condensin is required for chromosome partitioning and compaction. [More information is available at EcoGene: EG12165]. MukF plays a role in chromosome partitioning during cell division . [More information is available at EcoCyc: EG12165].
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    Genomic context

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    Sequence:
    NC_000913.3 (974319..975641)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF03966 family protein YcaR Neighboring gene 3-deoxy-manno-octulosonate cytidylyltransferase Neighboring gene putative phosphotransferase YcbJ Neighboring gene envelope biogenesis factor Neighboring gene tRNA cmo(5)U34 methyltransferase Neighboring gene chromosome partitioning protein MukE Neighboring gene chromosome partitioning protein MukB Neighboring gene L,D-transpeptidase LdtD

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin. Zawadzka K, et al. Elife, 2018 Jan 11. PMID 29323635, Free PMC Article
    2. MukE and MukF form two distinct high affinity complexes. Gloyd M, et al. J Biol Chem, 2007 May 11. PMID 17355972
    3. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation. Prince JP, et al. Nat Commun, 2021 Nov 18. PMID 34795302, Free PMC Article
    4. Comparison of MukB homodimer versus MukBEF complex molecular architectures by electron microscopy reveals a higher-order multimerization. Matoba K, et al. Biochem Biophys Res Commun, 2005 Aug 5. PMID 15979051
    5. The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins. Fennell-Fezzie R, et al. EMBO J, 2005 Jun 1. PMID 15902272, Free PMC Article

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
      Title: Intersubunit and intrasubunit interactions driving the MukBEF ATPase.
    2. Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
      Title: Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation.
    3. Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
      Title: Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
    4. Complex formation requires functional interactions between the C- and N-terminal domains of MukF with the MukB head and neck, respectively, and MukE, which organizes the complexes by stabilizing binding of MukB heads to MukF. In the absence of head engagement, a MukF dimer bound by MukE forms complexes containing only a dimer of MukB.
      Title: Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF.
    5. The authors show that a 4-helix bundle adjacent to the MukF dimerisation domain interacts functionally with the MukB coiled-coiled 'neck' adjacent to the ATPase head. They propose that this interaction leads to an asymmetric tripartite complex, as in other SMC (Structural Maintenance of Chromosomes) complexes.
      Title: MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.
    6. activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
      Title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
    7. MatP regulates the action of topoisomerase IV and MukBEF in chromosome segregation.
      Title: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.
    8. Taken together, the data implicate MukBEF as a key component of the DNA segregation process by acting in concert with TopoIV to promote decatenation and positioning of newly replicated oris.
      Title: The SMC complex MukBEF recruits topoisomerase IV to the origin of replication region in live Escherichia coli.
    9. MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors.
      Title: In vivo architecture and action of bacterial structural maintenance of chromosome proteins.
    10. findings show here that MukBEF generates and maintains the cellular positioning of chromosome loci independently of DNA replication.
      Title: The Escherichia coli SMC complex, MukBEF, shapes nucleoid organization independently of DNA replication.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome condensation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome condensation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in chromosome segregation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of condensin complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    chromosome partitioning protein MukF

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      974319..975641
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415442.1 chromosome partitioning protein MukF [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415442.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1IXE4, A0A2X1ND92, A0A9P2IB14
      Conserved Domains (1) summary
      PRK05260
      Location:1 → 440
      PRK05260; chromosome partition protein MukF
    Nucleotide Protein
    Heading Accession and Version

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