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    mnmA tRNA-specific 2-thiouridylase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945690, updated on 17-Dec-2024

    Summary

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    Official Symbol
    mnmA
    Official Full Name
    tRNA-specific 2-thiouridylase
    Primary source
    ECOCYC:EG11344
    Locus tag
    b1133
    See related
    ASAP:ABE-0003815
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    asuE; ECK1119; trmU; ycfB
    Summary
    Sulfur may be donated to tRNA U34 from YccK(TusD)-activated MnmA or from YccK(TusD) directly. [More information is available at EcoGene: EG11344]. MnmA catalyzes formation of the 2-thiouridine modification of tRNA(Glu) in vitro in the presence of Mg-ATP, cysteine, and the IscS cysteine desulfurase . [More information is available at EcoCyc: EG11344].
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    Genomic context

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    Sequence:
    NC_000913.3 (1192667..1193773, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene adenylosuccinate lyase Neighboring gene lysogenization regulator Neighboring gene phosphatase NudJ Neighboring gene 23S rRNA pseudouridine(2457) synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine. Sullivan MA, et al. J Bacteriol, 1985 Jan. PMID 3881393, Free PMC Article
    2. Aminoacylation of transfer RNAs with 2-thiouridine derivatives in the wobble position of the anticodon. Rogers KC, et al. Biochimie, 1995. PMID 7541255
    3. Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu). Numata T, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2006 Apr 1. PMID 16582487, Free PMC Article
    4. Expression of a coronavirus ribosomal frameshift signal in Escherichia coli: influence of tRNA anticodon modification on frameshifting. Brierley I, et al. J Mol Biol, 1997 Jul 18. PMID 9237903, Free PMC Article
    5. The cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar typhimurium. Nilsson K, et al. J Bacteriol, 2002 Dec. PMID 12446633, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The asymmetric units of these crystals are expected to contain two, one and two MnmA-tRNA(Glu) complexes, respectively.
      Title: Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu).
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sulfurtransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfurtransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA-uridine 2-sulfurtransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble position uridine thiolation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA wobble position uridine thiolation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA wobble position uridine thiolation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA wobble position uridine thiolation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of sulfurtransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA-specific 2-thiouridylase
    NP_415651.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1192667..1193773 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415651.4 tRNA-specific 2-thiouridylase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415651.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2X1NHL1
      Conserved Domains (1) summary
      PRK00143
      Location:6 → 361
      mnmA; tRNA-specific 2-thiouridylase MnmA; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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