ruvA Holliday junction branch migration complex subunit RuvA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: ruvA
Official Full Name: Holliday junction branch migration complex subunit RuvA
Primary source: ECOCYC:RUVA
Locus tag: b1861
See related: ASAP:ABE-0006206
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1862
Summary: Binds Holliday junction as two tetramers (an octamer) and each tetrameric half recruits one RuvB hexameric helicase. [More information is available at EcoGene: EG10923]. RuvABC is an enzymatic complex which functions in recombination pathways to by binding to recombinational junctions and catalyzing strand cleavage and branch migration. [More information is available at EcoCyc: RUVA].
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Genomic context

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Sequence:: NC_000913.3 (1945365..1945976, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regression of replication forks stalled by leading-strand template damage: both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
Title: Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
These results suggest that RecQ acts upstream of RuvABC, RecG and XerC proteins, a finding that is compatible with its primary role in initiation of the RecF recombination pathway.
Title: RecQ helicase acts before RuvABC, RecG and XerC proteins during recombination in recBCD sbcBC mutants of Escherichia coli.
RNA polymerase mutations reduce the synergism between priB and ruvABC.
Title: Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase.
DNA fragmentation absolutely depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction resolution
Title: Replication forks stalled at ultraviolet lesions are rescued via RecA and RuvABC protein-catalyzed disintegration in Escherichia coli.
Role in converting the initial single-strand DNA-protein cleavage complex into a double-strand break prior to repair by homologous recombination.
Title: Analysis of RuvABC and RecG involvement in the escherichia coli response to the covalent topoisomerase-DNA complex.
Study hypothesized that RuvAB catalyzes replication fork reversal in order to produce SOS(Con) expression.
Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
The authors present here the isolation and characterization of ruvA and ruvB single mutants that are impaired for replication fork reversal at forks arrested by the inactivation of polymerase III, while they remain capable of homologous recombination.
Title: ruvA and ruvB mutants specifically impaired for replication fork reversal.
RuvABC is required to resolve junctions that arise during the repair of a subset of nonarresting lesions after replication has passed through the template
Title: RuvABC is required to resolve holliday junctions that accumulate following replication on damaged templates in Escherichia coli.
RuvA octamerization is essential for the full biological activity of RuvABC.
Title: The role of RuvA octamerization for RuvAB function in vitro and in vivo.
RuvA is less mobile at a heterologous junction compared to a homologous junction, as two opposing DnaB pumps are required to mobilize RuvA over heterologous DNA.
Title: RuvA is a sliding collar that protects Holliday junctions from unwinding while promoting branch migration.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in SOS response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within SOS response	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within SOS response	RCA inferred from Reviewed Computational Analysis more info	PubMed
involved_in recombinational repair	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within recombinational repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in recombinational repair	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to radiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Holliday junction helicase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Holliday junction helicase complex	IEA Inferred from Electronic Annotation more info
part_of Holliday junction helicase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Holliday junction resolvase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Holliday junction resolvase complex	IEA Inferred from Electronic Annotation more info
part_of Holliday junction resolvase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Holliday junction branch migration complex subunit RuvA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1945365..1945976 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416375.1 Holliday junction branch migration complex subunit RuvA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416375.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376M7W0, A0AAN3TQB6

Conserved Domains (1) summary

PRK00116
Location:1 → 203

ruvA; Holliday junction branch migration protein RuvA