U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    nuoB NADH:quinone oxidoreductase subunit B [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946738, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    nuoB
    Official Full Name
    NADH:quinone oxidoreductase subunit B
    Primary source
    ECOCYC:EG12083
    Locus tag
    b2287
    See related
    ASAP:ABE-0007551
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2281
    Summary
    NuoB has iron-sulfur cluster N2. [More information is available at EcoGene: EG12083]. NuoB is part of the connecting fragment of NADH dehydrogenase I . [More information is available at EcoCyc: EG12083].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2403951..2404613, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADH:quinone oxidoreductase subunit F Neighboring gene NADH:quinone oxidoreductase subunit E Neighboring gene NADH:quinone oxidoreductase subunit CD Neighboring gene NADH:quinone oxidoreductase subunit A Neighboring gene DNA-binding transcriptional dual regulator LrhA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I). Flemming D, et al. J Mol Microbiol Biotechnol, 2005. PMID 16645316
    2. Involvement of tyrosines 114 and 139 of subunit NuoB in the proton pathway around cluster N2 in Escherichia coli NADH:ubiquinone oxidoreductase. Flemming D, et al. J Biol Chem, 2003 Jan 31. PMID 12446673
    3. Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Flemming D, et al. J Biol Chem, 2006 Aug 25. PMID 16807239
    4. Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I). Pohl T, et al. Biochim Biophys Acta, 2010 Dec. PMID 20959113
    5. Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus. Berrisford JM, et al. Biochemistry, 2008 Sep 30. PMID 18771280

    See all (65) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study determined the distance between a MTSL labeled complex I variant and the bound quinone by continuous-wave (cw) EPR allowing an inference on the location of the quinone binding site.
      Title: Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I).
    2. N-terminus verified by Edman degradation on complete protein
      Title: Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    contributes_to NADH dehydrogenase (ubiquinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NADH dehydrogenase (ubiquinone) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to NADH dehydrogenase (ubiquinone) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    contributes_to NADH dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NADH:ubiquinone reductase (non-electrogenic) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables quinone binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in aerobic respiration IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in aerobic respiration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in electron transport coupled proton transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in electron transport coupled proton transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in respiratory electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of NADH dehydrogenase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of respiratory chain complex I IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of respiratory chain complex I IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of respiratory chain complex I IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of respiratory chain complex I NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    NADH:quinone oxidoreductase subunit B
    NP_416790.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2403951..2404613 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416790.1 NADH:quinone oxidoreductase subunit B [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416790.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7H4YJW6
      Conserved Domains (1) summary
      COG0377
      Location:17 → 210
      NuoB; NADH:ubiquinone oxidoreductase 20 kD subunit (chhain B) or related Fe-S oxidoreductase [Energy production and conversion]
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.