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    rhlB ATP-dependent RNA helicase RhlB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948290, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rhlB
    Official Full Name
    ATP-dependent RNA helicase RhlB
    Primary source
    ECOCYC:EG10844
    Locus tag
    b3780
    See related
    ASAP:ABE-0012351
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3772; mmrA
    Summary
    RhlB is an ATP-dependent helicase that is a component of the degradosome, where it aids degradation of structured RNA by PNPase and is required for full degradosome activity . [More information is available at EcoCyc: EG10844].
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    Genomic context

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    Sequence:
    NC_000913.3 (3964365..3965630, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ATP-dependent DNA helicase Rep Neighboring gene guanosine-5'-triphosphate,3'-diphosphate phosphatase Neighboring gene thioredoxin 1 Neighboring gene rho operon leader peptide

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A mechanism for rich-medium inhibition of the repair of daughter-strand gaps in the deoxyribonucleic acid of UV-irradiated Escherichia coli K12 uvrA. Sharma RC, et al. Mutat Res, 1985 Sep. PMID 3897844
    2. Assaying DEAD-box RNA helicases and their role in mRNA degradation in Escherichia coli. Carpousis AJ, et al. Methods Enzymol, 2008. PMID 19161844
    3. New mutation (mmrA1) in Escherichia coli K-12 that affects minimal medium recovery and postreplication repair after UV irradiation. Sharma RC, et al. J Bacteriol, 1983 May. PMID 6341360, Free PMC Article
    4. Bacteriophage T7 protein kinase phosphorylates RNase E and stabilizes mRNAs synthesized by T7 RNA polymerase. Marchand I, et al. Mol Microbiol, 2001 Nov. PMID 11722741
    5. DEAD box RhlB RNA helicase physically associates with exoribonuclease PNPase to degrade double-stranded RNA independent of the degradosome-assembling region of RNase E. Liou GG, et al. J Biol Chem, 2002 Oct 25. PMID 12181321

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The association between Hfq and RNase E in long-term nitrogen-starved Escherichia coli.
      Title: The association between Hfq and RNase E in long-term nitrogen-starved Escherichia coli.
    2. The authors demonstrate that overexpression of RhlB partially inhibits the Hfq binding to RNase E and the rapid degradation of ptsG mRNA.
      Title: Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coli.
    3. RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome
      Title: RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.
    5. RhlB facilitates the degradation of ribosome-free mRNA by RNase E
      Title: Evidence in vivo that the DEAD-box RNA helicase RhlB facilitates the degradation of ribosome-free mRNA by RNase E.
    6. presented in vitro evidence that SrmB, RhlE and CsdA can bind Rnase E at a site that is different from the rhlB binding site; RhlE can replace the function of RhlB in vitro
      Title: The RNase E of Escherichia coli has at least two binding sites for DEAD-box RNA helicases: functional replacement of RhlB by RhlE.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables disordered domain specific binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within RNA catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in RNA catabolic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in RNA processing NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of bacterial degradosome IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ATP-dependent RNA helicase RhlB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3964365..3965630 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418227.1 ATP-dependent RNA helicase RhlB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418227.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3JAQ6
      Conserved Domains (1) summary
      PRK04837
      Location:2 → 420
      PRK04837; ATP-dependent RNA helicase RhlB; Provisional
    Nucleotide Protein
    Heading Accession and Version

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