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    metF 5,10-methylenetetrahydrofolate reductase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948432, updated on 3-Dec-2024

    Summary

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    Official Symbol
    metF
    Official Full Name
    5,10-methylenetetrahydrofolate reductase
    Primary source
    ECOCYC:EG10585
    Locus tag
    b3941
    See related
    ASAP:ABE-0012897
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3933
    Summary
    Upregulated by GSNO in defined medium. [More information is available at EcoGene: EG10585].
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    Genomic context

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    Sequence:
    NC_000913.3 (4132616..4133506)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene O-succinylhomoserine(thiol)-lyase/O-succinylhomoserine lyase Neighboring gene fused aspartate kinase/homoserine dehydrogenase 2 Neighboring gene catalase/hydroperoxidase HPI Neighboring gene cystine exporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. Sheppard CA, et al. J Bacteriol, 1999 Feb. PMID 9922232, Free PMC Article
    2. Role of the metF and metJ genes on the vitamin B12 regulation of methionine gene expression: involvement of N5-methyltetrahydrofolic acid. Cai XY, et al. Biochem Biophys Res Commun, 1992 Jan 31. PMID 1734876
    3. Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli. Stauffer GV, et al. Mol Gen Genet, 1988 May. PMID 2841568
    4. Operator-constitutive mutations of the Escherichia coli metF gene. Belfaiza J, et al. J Bacteriol, 1987 Feb. PMID 3542965, Free PMC Article
    5. Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcription unit. Treat ML, et al. Mol Gen Genet, 1984. PMID 6319973

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Results suggest that Leu may be a suitable replacement for Phe223 in the oxidative half-reaction of E. coli methylenetetrahydrofolate reductase.
      Title: Functional role for the conformationally mobile phenylalanine 223 in the reaction of methylenetetrahydrofolate reductase from Escherichia coli.
    2. The invariant aspartate 120 is a critical residue for E. coli methylenetetrahydrofolate reductase, with major roles in folate binding and catalysis, and a minor role in redox modulation.
      Title: Aspartate 120 of Escherichia coli methylenetetrahydrofolate reductase: evidence for major roles in folate binding and catalysis and a minor role in flavin reactivity.
    3. Multiple onformation, which allows aspartic acid 120 to hydrogen bond to the pterin ring in the folate complex but must move to an "open" conformation to allow NADH to bind.
      Title: Structures of NADH and CH3-H4folate complexes of Escherichia coli methylenetetrahydrofolate reductase reveal a spartan strategy for a ping-pong reaction.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FAD binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables FAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylenetetrahydrofolate reductase (NAD(P)H) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methylenetetrahydrofolate reductase (NAD(P)H) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylenetetrahydrofolate reductase (NAD(P)H) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylenetetrahydrofolate reductase (NADH) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-folding chaperone binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in methionine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in methionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tetrahydrofolate interconversion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tetrahydrofolate interconversion IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    5,10-methylenetetrahydrofolate reductase
    NP_418376.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4132616..4133506
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418376.1 5,10-methylenetetrahydrofolate reductase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418376.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAN5UFN7
      Conserved Domains (1) summary
      PRK09432
      Location:1 → 296
      metF; methylenetetrahydrofolate reductase
    Nucleotide Protein
    Heading Accession and Version

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