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    ybfF esterase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945288, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ybfF
    Official Full Name
    esterase
    Primary source
    ECOCYC:EG11776
    Locus tag
    b0686
    See related
    ASAP:ABE-0002337
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0674
    Summary
    Thioesterase activity of YbfF with large substrates such as palmitoyl-CoA and malonyl-CoA was discovered in a high-throughput screen of purified proteins . [More information is available at EcoCyc: EG11776].
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    Genomic context

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    Sequence:
    NC_000913.3 (712038..712802, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene flavodoxin 1 Neighboring gene ribbon-helix-helix domain-containing protein YbfE Neighboring gene negative modulator of initiation of replication Neighboring gene phosphoglucomutase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Discovery of an Escherichia coli esterase with high activity and enantioselectivity toward 1,2-O-isopropylideneglycerol esters. Godinho LF, et al. Appl Environ Microbiol, 2011 Sep. PMID 21764964, Free PMC Article
    2. Rapid functional identification of putative genes based on the combined in vitro protein synthesis with mass spectrometry: a tool for functional genomics. Kim JH, et al. Anal Biochem, 2008 Apr 1. PMID 18258174
    3. High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement. Park SY, et al. J Mol Biol, 2008 Mar 7. PMID 18215690
    4. Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Park SY, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Dec 1. PMID 18084091, Free PMC Article
    5. Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography. Fountoulakis M, et al. Electrophoresis, 1999 Aug. PMID 10493123

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This approach led to the identification of esterase YbfF as the major Escherichia coli enzyme responsible for the hydrolytic activity toward 1,2-O-isopropylideneglycerol esters.
      Title: Discovery of an Escherichia coli esterase with high activity and enantioselectivity toward 1,2-O-isopropylideneglycerol esters.
    2. The high-resolution structure of ybfF shows that the inserted helical bundle structure is critical for creating a large inner space, thereby providing the broad substrate spectrum toward large biomolecules.
      Title: High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement.
    3. With two Ec_ybfF molecules in the asymmetric unit, the crystal volume per unit protein weight is 2.17 A(3) Da(-1), corresponding to a solvent content of 42%
      Title: Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carboxylesterase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables carboxylic ester hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thiolester hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    esterase

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      712038..712802 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415212.1 esterase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415212.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7RTH0
      Conserved Domains (1) summary
      PRK10673
      Location:1 → 254
      PRK10673; esterase
    Nucleotide Protein
    Heading Accession and Version

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