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    pyrC dihydroorotase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945787, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pyrC
    Official Full Name
    dihydroorotase
    Primary source
    ECOCYC:EG10806
    Locus tag
    b1062
    See related
    ASAP:ABE-0003607
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1047
    Summary
    Dihydroorotase catalyzes the cyclization of carbamoyl-aspartate to dihydroorotate. [More information is available at EcoCyc: EG10806].
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    Genomic context

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    Sequence:
    NC_000913.3 (1121561..1122607, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene regulator of biofilm formation Neighboring gene DNA damage-inducible protein I Neighboring gene putative lipid-binding lipoprotein YceB Neighboring gene reduced glutaredoxin 2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center. Thoden JB, et al. Biochemistry, 2001 Jun 19. PMID 11401542
    2. Translational control of pyrC expression mediated by nucleotide-sensitive selection of transcriptional start sites in Escherichia coli. Wilson HR, et al. J Bacteriol, 1992 Jan. PMID 1345912, Free PMC Article
    3. Dihydroorotase from Escherichia coli. Substitution of Co(II) for the active site Zn(II). Brown DC, et al. J Biol Chem, 1991 Jan 25. PMID 1671037
    4. Dual transcriptional initiation sites from the pyrC promoter control expression of the gene in Salmonella typhimurium. Sørensen KI, et al. Mol Gen Genet, 1991 Feb. PMID 1706467
    5. Dihydroorotase from Escherichia coli. Cloning the pyrC gene and production of tryptic peptide maps. Brown DC, et al. J Biol Chem, 1986 May 5. PMID 2871019

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DAta show that expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively in the fur+ strain compared to those in the fur- strain.
      Title: Effect of fur on pyrC gene expression.
    2. we report the structures of Escherichia coli DHOase crystallized without ligand (1.7 A resolution) and in the presence of the inhibitors 2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate (2.0 A) and 5-fluoroorotate (2.2 A).
      Title: Structures of ligand-free and inhibitor complexes of dihydroorotase from Escherichia coli: implications for loop movement in inhibitor design.
    3. The kinetic and structural analyses suggest that Thr109 and Thr110 in the flexible loop provide productive binding of substrate and stabilize the transition-state intermediate, thereby increasing catalytic activity.
      Title: Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: a flexible loop stabilizes the transition state.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    5. Results show that a loop comprised of residues 105-115 of dihydroorotase has different conformations in its two subunits.
      Title: Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables dihydroorotase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables dihydroorotase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables dihydroorotase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in 'de novo' UMP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in 'de novo' pyrimidine nucleobase biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pyrimidine nucleobase biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pyrimidine nucleotide biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in pyrimidine nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    dihydroorotase
    NP_415580.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1121561..1122607 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415580.1 dihydroorotase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415580.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9ANG4
      Conserved Domains (1) summary
      PRK05451
      Location:4 → 347
      PRK05451; dihydroorotase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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