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    mcrA Type IV methyl-directed restriction enzyme EcoKMcrA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945727, updated on 3-Dec-2024

    Summary

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    Official Symbol
    mcrA
    Official Full Name
    Type IV methyl-directed restriction enzyme EcoKMcrA
    Primary source
    ECOCYC:EG10573
    Locus tag
    b1159
    See related
    ASAP:ABE-0003891
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1145; rglA
    Summary
    Purified McrA specifically binds to but does not cut HpaII methylated DNA (Mulligan, 2008). [More information is available at EcoGene: EG10573]. The McrA protein is a type IV site-specific deoxyribonuclease, which is one of three restriction systems defending the cell against foreign DNA, such as bacteriophages. [More information is available at EcoCyc: EG10573].
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    Genomic context

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    Sequence:
    NC_000913.3 (1210346..1211179)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative tail fiber assembly protein TfaE Neighboring gene pseudo Neighboring gene site-specific DNA recombinase Neighboring gene pseudo Neighboring gene anti-adaptor protein IraM

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Modification and restriction of T-even bacteriophages. In vitro degradation of deoxyribonucleic acid containing 5-hydroxymethylctosine. Fleischman RA, et al. J Biol Chem, 1976 Mar 25. PMID 767337
    2. Cloning, purification and initial characterization of E. coli McrA, a putative 5-methylcytosine-specific nuclease. Mulligan EA, et al. Protein Expr Purif, 2008 Nov. PMID 18662788, Free PMC Article
    3. Different mechanisms for SOS induced alleviation of DNA restriction in Escherichia coli. Hiom K, et al. Biochimie, 1991 Apr. PMID 1655051
    4. Cloning and structural characterization of the mcrA locus of Escherichia coli. Hiom K, et al. J Bacteriol, 1991 Nov. PMID 1938927, Free PMC Article
    5. Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease. Anton BP, et al. J Bacteriol, 2004 Sep. PMID 15317774, Free PMC Article

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyl-CpG binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA restriction-modification system IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Type IV methyl-directed restriction enzyme EcoKMcrA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1210346..1211179
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415677.1 Type IV methyl-directed restriction enzyme EcoKMcrA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415677.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P24200
      Conserved Domains (1) summary
      cd00085
      Location:194 → 256
      HNHc; HNH nucleases; HNH endonuclease signature which is found in viral, prokaryotic, and eukaryotic proteins. The alignment includes members of the large group of homing endonucleases, yeast intron 1 protein, MutS, as well as bacterial colicins, pyocins, and ...
    Nucleotide Protein
    Heading Accession and Version

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