U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    truA tRNA pseudouridine(38-40) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946793, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    truA
    Official Full Name
    tRNA pseudouridine(38-40) synthase
    Primary source
    ECOCYC:EG10454
    Locus tag
    b2318
    See related
    ASAP:ABE-0007657
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    asuC; ECK2312; hisT; leuK
    Summary
    [More information is available at EcoGene: EG10454]. TruA is the tRNA pseudouridine synthase responsible for catalyzing pseudouridine formation in the anticodon loop of a subset of tRNAs . [More information is available at EcoCyc: EG10454].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2434824..2435636, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene acetyl-CoA carboxyltransferase subunit beta Neighboring gene DedA family protein DedA Neighboring gene putative semialdehyde dehydrogenase Usg Neighboring gene erythronate-4-phosphate dehydrogenase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Biochemical characterization of an Escherichia coli hisT strain. Lawther RP, et al. J Bacteriol, 1977 Apr. PMID 192717, Free PMC Article
    2. Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. Bruni CB, et al. J Bacteriol, 1977 Apr. PMID 323237, Free PMC Article
    3. Absence of hisT-mediated tRNA pseudouridylation results in a uracil requirement that interferes with Escherichia coli K-12 cell division. Tsui HC, et al. J Bacteriol, 1991 Nov. PMID 1938930, Free PMC Article
    4. 1H-15N NMR studies of Escherichia coli tRNA(Phe) from hisT mutants: a structural role for pseudouridine. Davis DR, et al. Biochemistry, 1991 Apr 30. PMID 2021615
    5. Escherichia coli B/r leuK mutant lacking pseudouridine synthase I activity. Searles LL, et al. J Bacteriol, 1986 Apr. PMID 3514581, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. TruA displayed a rate constant of pseudouridine formation of 0.35 sec(-1).
      Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I.
    3. molecular basis for the site and substrate "promiscuity" was studied by determining the crystal structures of TruA in complex with two different leucyl tRNAs
      Title: How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity, acting on RNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine(38-40) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    tRNA pseudouridine(38-40) synthase
    NP_416821.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2434824..2435636 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416821.1 tRNA pseudouridine(38-40) synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416821.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6D0IGG0
      Conserved Domains (1) summary
      PRK00021
      Location:11 → 252
      truA; tRNA pseudouridine(38-40) synthase TruA
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.