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    rplE 50S ribosomal subunit protein L5 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947800, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rplE
    Official Full Name
    50S ribosomal subunit protein L5
    Primary source
    ECOCYC:EG10868
    Locus tag
    b3308
    See related
    ASAP:ABE-0010833
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3295
    Summary
    rplE is an essential gene as judged by the inability to recombineer haploid deletions (Baba, 2006; Korepanov, 2006). [More information is available at EcoGene: EG10868]. The L5 protein is a component of the 50S subunit of the ribosome. [More information is available at EcoCyc: EG10868].
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    Genomic context

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    Sequence:
    NC_000913.3 (3446899..3447438, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit protein S8 Neighboring gene 30S ribosomal subunit protein S14 Neighboring gene 50S ribosomal subunit protein L24 Neighboring gene 50S ribosomal subunit protein L14

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance. Korepanov AP, et al. Nucleic Acids Res, 2012 Oct. PMID 22821559, Free PMC Article
    2. Alteration of ribosomal protein L5 in a thiopeptin-resistant mutant of Escherichia coli. Liou YF, et al. Biochem Biophys Res Commun, 1975 Aug 4. PMID 1098662
    3. The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes. Chen R, et al. FEBS Lett, 1976 Oct 15. PMID 791672
    4. Co-operative binding of ribosomal proteins to an erythromycin affinity column. Pye DA, et al. Biochem Int, 1990 Aug. PMID 2256953
    5. The subunit interface of the Escherichia coli ribosome. Identification of proteins at the interface between the 30 S and 50 S subunits by crosslinking with 2-iminothiolane. Lambert JM, et al. J Mol Biol, 1981 Jul 5. PMID 7031269

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Demonstration that protein L5 plays a key role in formation of the central protuberance during assembly of the large ribosomal subunit in the bacterial cell.
      Title: Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5S rRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal large subunit assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic large ribosomal subunit IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic large ribosomal subunit IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in ribosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    50S ribosomal subunit protein L5

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3446899..3447438 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417767.1 50S ribosomal subunit protein L5 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417767.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9APG0, A0AAD2NYP4
      Conserved Domains (1) summary
      PRK00010
      Location:1 → 179
      rplE; 50S ribosomal protein L5; Validated
    Nucleotide Protein
    Heading Accession and Version

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