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    cpxR DNA-binding transcriptional dual regulator CpxR [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948404, updated on 3-Dec-2024

    Summary

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    Official Symbol
    cpxR
    Official Full Name
    DNA-binding transcriptional dual regulator CpxR
    Primary source
    ECOCYC:EG10020
    Locus tag
    b3912
    See related
    ASAP:ABE-0012774
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3905; yiiA
    Summary
    CpxR is the response regulator that mediates a response by activating the transcription of stress-combative genes (csgBA, csgDEFG, degP, and cpxP). [More information is available at EcoCyc: EG10020].
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    Genomic context

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    Sequence:
    NC_000913.3 (4104972..4105670, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-dehydro-3-deoxy-D-gluconate:H(+) symporter Neighboring gene 6-hydroxyaminopurine reductase Neighboring gene sensor histidine kinase CpxA Neighboring gene periplasmic protein CpxP Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. CpxR negatively regulates IncFII-replicon plasmid pEC011 conjugation by directly binding to multi-promoter regions. Jia Y, et al. Res Vet Sci, 2022 Dec 5. PMID 35809419
    2. The DNA-binding domain of the Escherichia coli CpxR two-component response regulator is constitutively active and cannot be fully attenuated by fused adjacent heterologous regulatory domains. Tapparel C, et al. Microbiology (Reading), 2006 Feb. PMID 16436431
    3. In vitro evidence that RNA Polymerase acetylation and acetyl phosphate-dependent CpxR phosphorylation affect cpxP transcription regulation. Lima BP, et al. FEMS Microbiol Lett, 2016 Mar. PMID 26790713, Free PMC Article
    4. Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli. Yamamoto K, et al. Biosci Biotechnol Biochem, 2006 Jul. PMID 16861804
    5. The deduced amino-acid sequence of the cloned cpxR gene suggests the protein is the cognate regulator for the membrane sensor, CpxA, in a two-component signal transduction system of Escherichia coli. Dong J, et al. Gene, 1993 Dec 22. PMID 8294007

    See all (84) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
      Title: The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
    2. CpxR negatively regulates IncFII-replicon plasmid pEC011 conjugation by directly binding to multi-promoter regions.
      Title: CpxR negatively regulates IncFII-replicon plasmid pEC011 conjugation by directly binding to multi-promoter regions.
    3. Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
      Title: Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
    4. Overall, these data demonstrate that NlpE acts as a sentinel for two important envelope biogenesis processes, namely, lipoprotein sorting and oxidative folding, and they further establish NlpE as a bona fide member of the CpxA-CpxR two-component system.
      Title: The Lipoprotein NlpE Is a Cpx Sensor That Serves as a Sentinel for Protein Sorting and Folding Defects in the Escherichia coli Envelope.
    5. Study found that the CpxRA system regulates the virulence properties of Avian pathogenic Escherichia coli. Adherence, invasiveness, motility, production of type 1 fimbriae and biofilm were negatively affected in the DeltacpxA mutant indicating that the CpxA is required for full manifestation of these phenotypes.
      Title: The CpxRA stress response system regulates virulence features of avian pathogenic Escherichia coli.
    6. The CpxAR system is widely considered to be responsible for sensing attachment, specifically to hydrophobic surfaces. Using both single-cell and population-level analyses, we confirm RcsCDB activation upon surface contact, but find that the CpxAR system is not activated, in contrast to what had earlier been reported. Thus, the role of CpxAR in surface sensing and initiation of biofilm formation should be reconsidered.
      Title: Reassessing the role of the Escherichia coli CpxAR system in sensing surface contact.
    7. Activation of CpxR upregulates the expression of genes encoding protein folding and chaperone factors and downregulates the expression of a number of envelope proteins/
      Title: Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections.
    8. X-ray structures of the CpxR reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer.
      Title: Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.
    9. study found that yqjA promoter activity was highest at alkaline pH. Increased activity of the yqjA promoter required both the transcriptional regulator CpxR, in agreement with previous results, and sodium or potassium salts at alkaline pH.
      Title: Cpx-dependent expression of YqjA requires cations at elevated pH.
    10. findings show that fine-tuning the Cpx stress response is critical to maintain cell wall homeostasis, affecting cell growth, morphology, division, and antibiotic resistance
      Title: Fine-Tuning of the Cpx Envelope Stress Response Is Required for Cell Wall Homeostasis in Escherichia coli.
    Submit: New GeneRIF Correction See all GeneRIFs (26)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphorelay response regulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphorelay response regulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of cell-substrate adhesion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-DNA complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator CpxR

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4104972..4105670 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418348.1 DNA-binding transcriptional dual regulator CpxR [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418348.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A776LT37
      Conserved Domains (1) summary
      PRK10955
      Location:1 → 232
      PRK10955; envelope stress response regulator transcription factor CpxR
    Nucleotide Protein
    Heading Accession and Version

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