U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    tufB translation elongation factor Tu 2 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948482, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    tufB
    Official Full Name
    translation elongation factor Tu 2
    Primary source
    ECOCYC:EG11037
    Locus tag
    b3980
    See related
    ASAP:ABE-0013020
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3971; kirT; pulT
    Summary
    Elongation factor Tu (EF-Tu) is the most abundant protein in Escherichia coli. [More information is available at EcoCyc: EG11037].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4175944..4177128)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-Gly Neighboring gene tRNA-Thr Neighboring gene Sec translocon subunit SecE Neighboring gene transcription termination/antitermination factor NusG

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Experimental Evolution of Escherichia coli Harboring an Ancient Translation Protein. Kacar B, et al. J Mol Evol, 2017 Mar. PMID 28233029, Free PMC Article
    2. The interface between Escherichia coli elongation factor Tu and aminoacyl-tRNA. Yikilmaz E, et al. Biochemistry, 2014 Sep 9. PMID 25094027, Free PMC Article
    3. A kirromycin resistant elongation factor EF-Tu from Escherichia coli contains a threonine instead of an alanine residue in position 375. Duisterwinkel FJ, et al. FEBS Lett, 1981 Aug 17. PMID 6793402
    4. Selective inhibition of transcription of the E. coli tufB operon by guanosine-5'-diphosphate-3'-diphosphate. Mizushima-Sugano J, et al. Mol Gen Genet, 1983. PMID 6343785
    5. tuf gene dosage effects on the intracellular concentration of EF-TuB. van der Meide PH, et al. Eur J Biochem, 1983 Feb 1. PMID 6337848

    See all (189) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we engineered an approximately 700-million-year-old inferred ancestral variant of tufB, an essential gene encoding elongation factor Tu, and inserted it in a modern Escherichia coli genome in place of the native tufB gene. We subsequently evolved replicate hybrid bacterial populations for 2000 generations and examined the adaptive response via fitness assays, whole genome sequencing, proteomics, and biochemical assays.
      Title: Experimental Evolution of Escherichia coli Harboring an Ancient Translation Protein.
    2. Comparison of DeltaDeltaG(o) values for binding EF-Tu to aa-tRNA show that the majority of the interface residues stabilize the ternary complex and their thermodynamic contribution can depend on the tRNA species that is used.
      Title: The interface between Escherichia coli elongation factor Tu and aminoacyl-tRNA.
    3. Fusidic acid increases traps the ribosome EF-G complex in a preexisting conformation formed during translation. Only the 0.3 state is observed when poly(Phe) synthesis is prevented by omission of EF-Tu.
      Title: Single-molecule structural dynamics of EF-G--ribosome interaction during translocation.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    5. Ecdomains 2+3 suppressed the GTPase activity of the E coli domain 1, whereas those of Bacillut thuringensis EF-Tu stimulated the Bstdomain 1 GTPase.
      Title: Opposite roles of domains 2+3 of Escherichia coli EF-Tu and Bacillus stearothermophilus EF-Tu in the regulation of EF-Tu GTPase activity.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanosine tetraphosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables translation elongation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation elongation factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational elongation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translational elongation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational elongation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of guanyl-nucleotide exchange factor complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    translation elongation factor Tu 2

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4175944..4177128
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418407.1 translation elongation factor Tu 2 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418407.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9ASQ8
      Conserved Domains (1) summary
      PRK00049
      Location:1 → 393
      PRK00049; elongation factor Tu; Reviewed
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.