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    aphA acid phosphatase/phosphotransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948562, updated on 3-Dec-2024

    Summary

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    Official Symbol
    aphA
    Official Full Name
    acid phosphatase/phosphotransferase
    Primary source
    ECOCYC:EG11934
    Locus tag
    b4055
    See related
    ASAP:ABE-0013283
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4047; hobH; yjbP
    Summary
    Mutants arrest cell division after hyperosmoshock and overexpress SeqA; also binds hemi-methylated oriC DNA in vitro. [More information is available at EcoGene: EG11934]. Escherichia coli contains a periplasmic acid phosphatase/phosphotransferase. [More information is available at EcoCyc: EG11934].
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    Genomic context

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    Sequence:
    NC_000913.3 (4269414..4270127)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tyrosine aminotransferase Neighboring gene uncharacterized protein YjbS Neighboring gene PF01894 family protein YjbQ Neighboring gene PF04237 family protein YjbR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases. Leone R, et al. J Mol Biol, 2008 Dec 12. PMID 18845157
    2. Identification of an Escherichia coli periplasmic acid phosphatase containing of a 27 kDa-polypeptide component. Rossolini GM, et al. FEMS Microbiol Lett, 1994 May 1. PMID 8013875
    3. Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli. Thaller MC, et al. FEMS Microbiol Lett, 1999 Dec 1. PMID 10564784
    4. Expression, purification, crystallization and preliminary X-ray characterization of the class B acid phosphatase (AphA) from Escherichia coli. Forleo C, et al. Acta Crystallogr D Biol Crystallogr, 2003 Jun. PMID 12777773
    5. Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655. Passariello C, et al. Biochim Biophys Acta, 2006 Jan. PMID 16297670

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The present work reports the crystal structures of AphA from E. coli in complex with substrate, transition-state, and intermediate analogues.
      Title: Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases.
    2. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source.
      Title: Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-phosphoserine phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables acid phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables acid phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    acid phosphatase/phosphotransferase
    NP_418479.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4269414..4270127
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418479.1 acid phosphatase/phosphotransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418479.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0G4R1
      Conserved Domains (1) summary
      PRK11009
      Location:1 → 237
      aphA; class B acid phosphatase
    Nucleotide Protein
    Heading Accession and Version

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