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    ssb ssDNA-binding protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948570, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ssb
    Official Full Name
    ssDNA-binding protein
    Primary source
    ECOCYC:EG10976
    Locus tag
    b4059
    See related
    ASAP:ABE-0013298
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4051; exrB; lexC
    Summary
    SSB binds to RecQ and stimulates DNA unwinding (Shereda, 2007). [More information is available at EcoGene: EG10976]. The product of the essential ssb gene, the highly stable single-stranded DNA binding protein SSB, is involved in replication, recombination, and repair in the DNA metabolism of Escherichia coli. [More information is available at EcoCyc: EG10976].
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    Genomic context

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    Sequence:
    NC_000913.3 (4274125..4274661)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF04237 family protein YjbR Neighboring gene UvrABC excision nuclease subunit A Neighboring gene uncharacterized protein YjcB Neighboring gene c-di-GMP phosphodiesterase PdeC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Escherichia coli single-stranded DNA-binding protein, a molecular tool for improved sequence quality in pyrosequencing. Ehn M, et al. Electrophoresis, 2002 Sep. PMID 12373756
    2. Characterization of the structural and functional defect in the Escherichia coli single-stranded DNA binding protein encoded by the ssb-1 mutant gene. Expression of the ssb-1 gene under lambda pL regulation. Williams KR, et al. J Biol Chem, 1984 Oct 10. PMID 6384214
    3. The product of the lexC gene of Escherichia coli is single-stranded DNA-binding protein. Meyer RR, et al. J Bacteriol, 1982 Apr. PMID 7037753, Free PMC Article
    4. SSB functions as a sliding platform that migrates on DNA via reptation. Zhou R, et al. Cell, 2011 Jul 22. PMID 21784244, Free PMC Article
    5. Mechanism of Exonuclease I stimulation by the single-stranded DNA-binding protein. Lu D, et al. Nucleic Acids Res, 2011 Aug. PMID 21572106, Free PMC Article

    See all (154) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DNA damage alters binding conformations of E. coli single-stranded DNA-binding protein.
      Title: DNA damage alters binding conformations of E. coli single-stranded DNA-binding protein.
    2. RecA and SSB genome-wide distribution in ssDNA gaps and ends in Escherichia coli.
      Title: RecA and SSB genome-wide distribution in ssDNA gaps and ends in Escherichia coli.
    3. Interaction with the carboxy-terminal tip of SSB is critical for RecG function in E. coli.
      Title: Interaction with the carboxy-terminal tip of SSB is critical for RecG function in E. coli.
    4. Mutational Analysis of Residues in PriA and PriC Affecting Their Ability To Interact with SSB in Escherichia coli K-12.
      Title: Mutational Analysis of Residues in PriA and PriC Affecting Their Ability To Interact with SSB in Escherichia coli K-12.
    5. DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication forks in Escherichia coli.
      Title: DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication forks in Escherichia coli.
    6. Allosteric effects of SSB C-terminal tail on assembly of E. coli RecOR proteins.
      Title: Allosteric effects of SSB C-terminal tail on assembly of E. coli RecOR proteins.
    7. Multiprotein E. coli SSB-ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions.
      Title: Multiprotein E. coli SSB-ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions.
    8. RecA-SSB interaction should be included in discussing RecA regulatory mechanism.
      Title: RecA-SSB Interaction Modulates RecA Nucleoprotein Filament Formation on SSB-Wrapped DNA.
    9. SSB-AlkB interaction promotes faster repair of the methyl DNA adducts.
      Title: Escherichia coli single-stranded DNA binding protein SSB promotes AlkB-mediated DNA dealkylation repair.
    10. ExoI is primarily responsible for more efficiently abort template-switches on the lagging strand and that its ability to be recruited by single-strand DNA binding protein plays a critical role in quasipalindrome-associated mutagenesis avoidance and strand bias.
      Title: SSB recruitment of Exonuclease I aborts template-switching in Escherichia coli.
    Submit: New GeneRIF Correction See all GeneRIFs (42)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables enzyme activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA unwinding involved in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in SOS response TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in mismatch repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in recombinational repair TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleoid IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of single-stranded DNA-binding protein complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ssDNA-binding protein

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4274125..4274661
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418483.1 ssDNA-binding protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418483.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0G4Q5
      Conserved Domains (1) summary
      PRK09010
      Location:1 → 178
      PRK09010; single-stranded DNA-binding protein; Provisional
    Nucleotide Protein
    Heading Accession and Version

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