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    tilS tRNA(Ile)-lysidine synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944889, updated on 3-Dec-2024

    Summary

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    Official Symbol
    tilS
    Official Full Name
    tRNA(Ile)-lysidine synthetase
    Primary source
    ECOCYC:G6096
    Locus tag
    b0188
    See related
    ASAP:ABE-0000638
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0187; mesJ; yaeN
    Summary
    YaeN (TilS) is mistakenly labeled as a cell cycle gene in many databases. the confusion arose from the misinterpretation of Genbank Z50870. the real "minE suppressor" is the small rof gene next to tilS, labeled as orf4 in Z50870. [More information is available at EcoGene: EG13220]. TilS is a tRNAIle-lysidine synthetase, the enzyme responsible for modifying the wobble-base of the CAU anticodon of tRNAIle such that it exhibits proper recognition of the AUA codon rather than the AUG codon . [More information is available at EcoCyc: G6096].
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    Genomic context

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    Sequence:
    NC_000913.3 (212331..213629)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene lysine decarboxylase 2 Neighboring gene VOC domain-containing protein YaeR Neighboring gene modulator of Rho-dependent transcription termination Neighboring gene PF06786 family protein YaeP Neighboring gene uncharacterized protein YaeQ Neighboring gene peptidyl-tRNA hydrolase, ribosome rescue factor

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Discovery and characterization of tRNAIle lysidine synthetase (TilS). Suzuki T, et al. FEBS Lett, 2010 Jan 21. PMID 19944692
    2. Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase. Nakanishi K, et al. Nature, 2009 Oct 22. PMID 19847269
    3. Differential annotation of tRNA genes with anticodon CAT in bacterial genomes. Silva FJ, et al. Nucleic Acids Res, 2006. PMID 17071718, Free PMC Article
    4. molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition. Ikeuchi Y, et al. Mol Cell, 2005 Jul 22. PMID 16039592
    5. Enzymatic conversion of cytidine to lysidine in anticodon of bacterial isoleucyl-tRNA--an alternative way of RNA editing. Grosjean H, et al. Trends Biochem Sci, 2004 Apr. PMID 15124629

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity, forming carbon-nitrogen bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA(Ile)-lysidine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA(Ile)-lysidine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within tRNA modification IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA wobble base lysidine biosynthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA(Ile)-lysidine synthetase
    NP_414730.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      212331..213629
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414730.1 tRNA(Ile)-lysidine synthetase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414730.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N8NGM9
      Conserved Domains (1) summary
      PRK10660
      Location:1 → 432
      tilS; tRNA(Ile)-lysidine synthetase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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