tolB Tol-Pal system periplasmic protein TolB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: tolB
Official Full Name: Tol-Pal system periplasmic protein TolB
Primary source: ECOCYC:EG11008
Locus tag: b0740
See related: ASAP:ABE-0002517
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0729; lky; lkyA; tol-3
Summary: Mutants have elevated OM vesiculation (McBroom, 2006). [More information is available at EcoGene: EG11008]. TolB is a periplasmic protein member of the YbgC-YbgF-TolQ-R-A-B-Pal Cell Envelope Complex, also known as the Tol-Pal system . [More information is available at EcoCyc: EG11008].
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Genomic context

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Sequence:: NC_000913.3 (777740..779032)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppressor mutations in degS overcome the acute temperature-sensitive phenotype of DeltadegP and DeltadegP Deltatol-pal mutants of Escherichia coli.
Title: Suppressor Mutations in degS Overcome the Acute Temperature-Sensitive Phenotype of ΔdegP and ΔdegP Δtol-pal Mutants of Escherichia coli.
Colicin N and M13 phage protein G3p compete with TolB for a binding site on TolA.
Title: Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA.
Using a combination of in vivo coimmunoprecipitations and in vitro pulldown experiments, we demonstrated that KT interacts with the TolA, TolB, and TolR proteins.
Title: Interaction of the colicin K bactericidal toxin with components of its import machinery in the periplasm of Escherichia coli.
The N-terminal 107 residues of ColA (TA1-107) bound to TolB to show that the TolB box of ColA consists of a 12-residue domain that binds to the beta-propeller canyon of TolB.
Title: The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
analysis of allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins
Title: Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins.
N-terminus verified by Edman degradation on mature peptide
Title: '98 Escherichia coli SWISS-2DPAGE database update.
the TolB box sequence in ColE9 is actually larger than the pentapeptide and extends from residues 34 to 46
Title: Interactions of TolB with the translocation domain of colicin E9 require an extended TolB box.
explains how colicins recruit TolB in the bacterial periplasm and highlights a novel binding mechanism for a natively disordered protein
Title: Molecular mimicry enables competitive recruitment by a natively disordered protein.
Results identify the 16-residue TolB binding epitope in the natively disordered T-domain of the nuclease colicin E9 (ColE9) and present the crystal structure of the complex.
Title: Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within bacteriocin transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell division	EXP Inferred from Experiment more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell division	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within cell septum assembly	EXP Inferred from Experiment more info	PubMed
acts_upstream_of_or_within cellular response to bacteriocin	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein import	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of membrane invagination	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cell division site	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	NAS Non-traceable Author Statement more info	PubMed
located_in outer membrane-bounded periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic space	IDA Inferred from Direct Assay more info	PubMed
located_in periplasmic space	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed