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    csgE curli assembly component CsgE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945711, updated on 3-Dec-2024

    Summary

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    Official Symbol
    csgE
    Official Full Name
    curli assembly component CsgE
    Primary source
    ECOCYC:G6545
    Locus tag
    b1039
    See related
    ASAP:ABE-0003528
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1025
    Summary
    Probable periplasmic protein with predicted 22 aa signal. [More information is available at EcoGene: EG13411]. An insertion in the csgE gene abolishes the formation of curli, although csgA, which encodes one of the structural subunits of curli, continues to be expressed. [More information is available at EcoCyc: G6545].
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    Genomic context

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    Sequence:
    NC_000913.3 (1102152..1102541, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene curli secretion channel Neighboring gene curli assembly component CsgF Neighboring gene DNA-binding transcriptional dual regulator CsgD Neighboring gene curlin, minor subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation. Shu Q, et al. Proc Natl Acad Sci U S A, 2016 Jun 28. PMID 27298344, Free PMC Article
    2. Continuous and pulsed hydrogen-deuterium exchange and mass spectrometry characterize CsgE oligomerization. Wang H, et al. Biochemistry, 2015 Oct 27. PMID 26418947, Free PMC Article
    3. Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG. Goyal P, et al. Nature, 2014 Dec 11. PMID 25219853, Free PMC Article
    4. Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation. Klein RD, et al. mBio, 2018 Jul 17. PMID 30018113, Free PMC Article
    5. Structural insights into functional amyloid inhibition in Gram -ve bacteria. Hawthorne W, et al. Biochem Soc Trans, 2016 Dec 15. PMID 27913673, Free PMC Article

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A region of positive charge formed by the confluence of the beta1-beta2 and beta3-alpha2 loops at the head of Curli transport specificity factor CsgE plays a key role in the CsgE-CsgA (amyloidogenic CsgA) interaction and is required for curli assembly. These mechanistic insights into the directed assembly of functional amyloids in extracellular biofilms elucidate possible targets for biofilm-associated bacterial infect...
      Title: Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation.
    2. The electro-negative and flexible tail of CsgE can adjust toward the electro-positive patches on the CsgG interface as the initial step in binding.
      Title: Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
    3. Studies show the common structural features of CsgC and CsgE and the potential implications for general inhibition of amyloid.
      Title: Structural insights into functional amyloid inhibition in Gram -ve bacteria.
    4. determined regions involved in CsgE self-association and on the basis of that, prepared a double mutant W48A/F79A, derived from interface alanine scan, and verified that it exists as a monomer
      Title: Continuous and pulsed hydrogen-deuterium exchange and mass spectrometry characterize CsgE oligomerization.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within curli assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in curli assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within protein secretion by the type VIII secretion system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein secretion by the type VIII secretion system NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein transmembrane transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in single-species biofilm formation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of curli secretion complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of curli secretion complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    curli assembly component CsgE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1102152..1102541 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415557.1 curli assembly component CsgE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415557.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FXW2
      Conserved Domains (1) summary
      PRK10386
      Location:1 → 129
      PRK10386; curli production assembly/transport protein CsgE
    Nucleotide Protein
    Heading Accession and Version

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