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    lsrK autoinducer-2 kinase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946069, updated on 3-Dec-2024

    Summary

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    Official Symbol
    lsrK
    Official Full Name
    autoinducer-2 kinase
    Primary source
    ECOCYC:G6798
    Locus tag
    b1511
    See related
    ASAP:ABE-0005041
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1504; ydeV
    Summary
    LsrK phosphorylates AI-2; phospo-AI-2 is retained in the cytoplasm. lsr operon induction by exogenous AI-2 requires LsrK, indicating phospho-AI-2, or a derivative, is the LsrR inducer. [More information is available at EcoGene: EG13804]. Based on its similarity to LsrK from Salmonella typhimurium , LsrK is predicted to phosphorylate the quorum-sensing autoinducer molecule AI-2 (shown for S. t. in ). [More information is available at EcoCyc: G6798].
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    Genomic context

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    Sequence:
    NC_000913.3 (1598617..1600209, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene serine/threonine-protein kinase toxin HipA Neighboring gene antitoxin/DNA-binding transcriptional repressor HipB Neighboring gene pseudo Neighboring gene DNA-binding transcriptional repressor LsrR Neighboring gene Autoinducer-2 ABC transporter ATP binding subunit Neighboring gene Autoinducer-2 ABC transporter membrane subunit LsrC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule. Rhoads MK, et al. Biotechnol Bioeng, 2018 Feb. PMID 28782813
    2. Identification of a novel Bcl-2 related gene, BRAG-1, in human glioma. Das R, et al. Oncogene, 1996 Mar 7. PMID 8649811
    3. Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2. Bansal T, et al. Appl Microbiol Biotechnol, 2008 Apr. PMID 18256823
    4. Cross species quorum quenching using a native AI-2 processing enzyme. Roy V, et al. ACS Chem Biol, 2010 Feb 19. PMID 20025244
    5. Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain. Zhu J, et al. Infect Immun, 2011 Apr. PMID 21300771, Free PMC Article

    See all (22) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. LsrK was expressed and purified from E. coli with linker tags containing tyrosine or glutamine residues, attached to a chitosan film and a capsule, and used in quorum sensing studies.
      Title: Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule.
    2. The soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK has been described.
      Title: Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK.
    3. These results suggest the involvement of AI-2-mediated quorum sensing in K88(+) Enterotoxigenic Escherichia coli
      Title: Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain.
    4. Culture supernatants of E. coli and Staphylococcus aureus luxS mutants were found not to contain detectable levels of AI-2 activity.
      Title: LuxS-independent formation of AI-2 from ribulose-5-phosphate.
    5. AI-2-mediated regulation of enterohemorrhagic Escherichia coli chemotaxis and colonization, as well as temporal regulation of EHEC transcriptome by AI-2.
      Title: Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables autoinducer-2 kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables autoinducer-2 kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables autoinducer-2 kinase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in quorum sensing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in quorum sensing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within single-species biofilm formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    autoinducer-2 kinase
    NP_416028.3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1598617..1600209 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416028.3 autoinducer-2 kinase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416028.3

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A830SLC9, A0A8T5YEQ2
      Conserved Domains (1) summary
      PRK10939
      Location:10 → 530
      PRK10939; autoinducer-2 (AI-2) kinase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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