nadE NH3-dependent NAD(+) synthetase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Official Symbol: nadE
Official Full Name: NH3-dependent NAD(+) synthetase
Primary source: ECOCYC:EG10663
Locus tag: b1740
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1738; efg; ntrL
Summary: NadE is regulated by cyaR sRNA (De Lay, 2009). [More information is available at EcoGene: EG10663]. NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. [More information is available at EcoCyc: EG10663].
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NC_000913.3 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

N-terminus verified by Edman degradation on complete protein
Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
x-ray crystallographic study of Escherichia coli NAD synthetase
Title: Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables NAD+ synthase (glutamine-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables NAD+ synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables NAD+ synthase activity	IEA Inferred from Electronic Annotation more info
enables glutaminase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within 'de novo' NAD biosynthetic process from aspartate	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within 'de novo' NAD biosynthetic process from aspartate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
involved_in NAD biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within NAD salvage	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within NAD salvage	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

NP_416254.1

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The following sections contain reference sequences that belong to a specific genome build. Explain