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    trmJ tRNA Cm32/Um32 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948610, updated on 17-Dec-2024

    Summary

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    Official Symbol
    trmJ
    Official Full Name
    tRNA Cm32/Um32 methyltransferase
    Primary source
    ECOCYC:G7327
    Locus tag
    b2532
    See related
    ASAP:ABE-0008332
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2529; yfhQ
    Summary
    TrmJ is the tRNA:Cm32/Um32 methyltransferase which introduces methyl groups at the 2'-O position of C32 and U32 of several tRNAs in vitro. [More information is available at EcoCyc: G7327].
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    Genomic context

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    Sequence:
    NC_000913.3 (2662583..2663323, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene cysteine desulfurase IscS Neighboring gene DNA-binding transcriptional dual regulator IscR Neighboring gene Nus factor SuhB Neighboring gene putative peptidase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily. Liu RJ, et al. Nucleic Acids Res, 2015 Sep 3. PMID 26202969, Free PMC Article
    2. Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster. Nakamura M, et al. J Biochem, 1999 Jul. PMID 10393315
    3. Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. Yamamoto Y, et al. DNA Res, 1997 Apr 28. PMID 9205837
    4. The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase. Purta E, et al. BMC Mol Biol, 2006 Jul 18. PMID 16848900, Free PMC Article
    5. Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth. Lopez-Campistrous A, et al. Mol Cell Proteomics, 2005 Aug. PMID 15911532

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA nucleoside ribose methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA nucleoside ribose methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA Cm32/Um32 methyltransferase
    NP_417027.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2662583..2663323 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417027.1 tRNA Cm32/Um32 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417027.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9APK7
      Conserved Domains (1) summary
      PRK15114
      Location:1 → 244
      PRK15114; tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ; Provisional
    Nucleotide Protein
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