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    dnaG DNA primase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947570, updated on 3-Dec-2024

    Summary

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    Official Symbol
    dnaG
    Official Full Name
    DNA primase
    Primary source
    ECOCYC:EG10239
    Locus tag
    b3066
    See related
    ASAP:ABE-0010065
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dnaP; ECK3056; parB; sdgA
    Summary
    DNA primase catalyzes the synthesis of RNA primers on single-stranded template DNA . [More information is available at EcoCyc: EG10239].
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    Genomic context

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    Sequence:
    NC_000913.3 (3211107..3212852)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene N(6)-L-threonylcarbamoyladenine synthase, TsaD subunit Neighboring gene 30S ribosomal subunit protein S21 Neighboring gene RNA polymerase sigma factor RpoD Neighboring gene stationary phase mismatch/uracil DNA glycosylase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Initiation of DNA replication by the dnaG protein. Benz EW Jr, et al. J Biol Chem, 1980 Feb 10. PMID 6985903
    2. Initiation of DNA replication by the Escherichia coli dnaG protein: evidence that tertiary structure is involved. Sims J, et al. Proc Natl Acad Sci U S A, 1980 Feb. PMID 6244591, Free PMC Article
    3. Expression, purification, crystallization, and NMR studies of the helicase interaction domain of Escherichia coli DnaG primase. Loscha K, et al. Protein Expr Purif, 2004 Feb. PMID 14711519
    4. Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis. Lupski JR, et al. Mol Gen Genet, 1982. PMID 6283308
    5. Affinity and sequence specificity of DNA binding and site selection for primer synthesis by Escherichia coli primase. Khopde S, et al. Biochemistry, 2002 Dec 17. PMID 12475230

    See all (113) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Metabolic cofactors NADH and FAD act as non-canonical initiating substrates for a primase and affect replication primer processing in vitro.
      Title: Metabolic cofactors NADH and FAD act as non-canonical initiating substrates for a primase and affect replication primer processing in vitro.
    2. we propose that DnaC controls the ability of DnaB to interact with primase by modifying the conformation of the N-terminal domain of DnaB
      Title: DnaC, the indispensable companion of DnaB helicase, controls the accessibility of DnaB helicase by primase.
    3. Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other.
      Title: Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other.
    4. This study identifies the C-terminal helicase-binding domain of DnaG primase (DnaG-C) and the highly conserved C-terminal region of single-stranded DNA-binding protein as interaction sites.
      Title: The helicase-binding domain of Escherichia coli DnaG primase interacts with the highly conserved C-terminal region of single-stranded DNA-binding protein.
    5. ParB complexes P1 plasmids, ParA-ATP stimulates this interaction and ParA-ADP inhibits ParB pairing activity in a parS-independent manner.
      Title: ParAB-mediated intermolecular association of plasmid P1 parS sites.
    6. A single-molecule analysis of the replication kinetics with varying DnaG concentrations indicates that a cooperative binding of two or three DnaG monomers to DnaB halts synthesis by Pol III.
      Title: Single-molecule studies of fork dynamics in Escherichia coli DNA replication.
    7. The structure reveals an unanticipated binding site that engages nucleic acid in two distinct configurations, indicating that it serves as a nonspecific capture and tracking locus for template DNA.
      Title: Identification of a DNA primase template tracking site redefines the geometry of primer synthesis.
    8. N-terminus verified by Edman degradation on mature peptide
      Title: Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.
    9. analysis of centromere pairing by a plasmid-encoded type I ParB protein
      Title: Centromere pairing by a plasmid-encoded type I ParB protein.
    10. essential for IncP-9 plasmid pM3 replication
      Title: Ability of IncP-9 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA primase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA primase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA primase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA replication, synthesis of primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, synthesis of primer IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA unwinding involved in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in replication fork processing NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA-directed RNA polymerase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of DnaB-DnaG complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of primosome complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of primosome complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA primase
    NP_417538.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3211107..3212852
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417538.1 DNA primase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417538.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5C9AFV3
      Conserved Domains (1) summary
      PRK05667
      Location:2 → 581
      dnaG; DNA primase; Validated
    Nucleotide Protein
    Heading Accession and Version

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