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    lptB lipopolysaccharide transport system ATP binding protein LptB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947725, updated on 3-Dec-2024

    Summary

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    Official Symbol
    lptB
    Official Full Name
    lipopolysaccharide transport system ATP binding protein LptB
    Primary source
    ECOCYC:EG11680
    Locus tag
    b3201
    See related
    ASAP:ABE-0010512
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3190; yhbG
    Summary
    LptB depletion results in rifampicin/bile salts sensitivity, indicative of an outer membrane integrity defect. [More information is available at EcoGene: EG11680]. LptB is an ATP-binding component of the LptAB-YrbK ABC transporter. [More information is available at EcoCyc: EG11680].
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    Genomic context

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    Sequence:
    NC_000913.3 (3343944..3344669)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Neighboring gene lipopolysaccharide transport system protein LptC Neighboring gene lipopolysaccharide transport system protein LptA Neighboring gene RNA polymerase sigma factor RpoN Neighboring gene ribosome hibernation-promoting factor

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB(2) FGC transporter through a rigid-body mechanism to extract LPS. Lundstedt EA, et al. Mol Microbiol, 2020 Aug. PMID 32236984, Free PMC Article
    2. Structural and functional studies of conserved nucleotide-binding protein LptB in lipopolysaccharide transport. Wang Z, et al. Biochem Biophys Res Commun, 2014 Sep 26. PMID 25172661
    3. Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport. Sherman DJ, et al. Proc Natl Acad Sci U S A, 2014 Apr 1. PMID 24639492, Free PMC Article
    4. The transmembrane α-helix of LptC participates in LPS extraction by the LptB(2) FGC transporter. Wilson A, et al. Mol Microbiol, 2022 Jul. PMID 35678757, Free PMC Article
    5. Identification of Residues in the Lipopolysaccharide ABC Transporter That Coordinate ATPase Activity with Extractor Function. Simpson BW, et al. mBio, 2016 Oct 18. PMID 27795402, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The transmembrane alpha-helix of LptC participates in LPS extraction by the LptB2 FGC transporter.
      Title: The transmembrane α-helix of LptC participates in LPS extraction by the LptB(2) FGC transporter.
    2. LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB2 FGC transporter through a rigid-body mechanism to extract LPS.
      Title: LptB-LptF coupling mediates the closure of the substrate-binding cavity in the LptB(2) FGC transporter through a rigid-body mechanism to extract LPS.
    3. Taken together, these data identify a specific binding site in LptB for the coupling helices of LptFG that is responsible for coupling of ATP hydrolysis by LptB with LptFG function to achieve lipopolysaccharide extraction.
      Title: Identification of Residues in the Lipopolysaccharide ABC Transporter That Coordinate ATPase Activity with Extractor Function.
    4. The two protomers of LptB have to work together for ATP hydrolysis and LPS transport.
      Title: Structural and functional studies of conserved nucleotide-binding protein LptB in lipopolysaccharide transport.
    5. both LptF and LptG formed a complex with LptB.
      Title: Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides.
    6. results suggest that LptB operate in the LPS assembly pathway and, together with other as-yet-unidentified components, could be part of a complex devoted to the transport of LPS from the periplasmic surface of the IM to the OM.
      Title: Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli.
    7. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Gram-negative-bacterium-type cell outer membrane assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in lipopolysaccharide transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within lipopolysaccharide transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of ATP-binding cassette (ABC) transporter complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of ATP-binding cassette (ABC) transporter complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of ATP-binding cassette (ABC) transporter complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transporter complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    lipopolysaccharide transport system ATP binding protein LptB
    NP_417668.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3343944..3344669
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417668.1 lipopolysaccharide transport system ATP binding protein LptB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417668.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376VWU5
      Conserved Domains (1) summary
      PRK10895
      Location:1 → 241
      PRK10895; lipopolysaccharide ABC transporter ATP-binding protein; Provisional
    Nucleotide Protein
    Heading Accession and Version

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