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    rpoA RNA polymerase subunit alpha [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947794, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rpoA
    Official Full Name
    RNA polymerase subunit alpha
    Primary source
    ECOCYC:EG10893
    Locus tag
    b3295
    See related
    ASAP:ABE-0010802
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3282; pez; phs; sez
    Summary
    HT_Cmplx11_Mem: [More information is available at EcoGene: EG10893]. RpoA is the subunit of the RNA polymerase core enzyme. [More information is available at EcoCyc: EG10893].
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    Genomic context

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    Sequence:
    NC_000913.3 (3440040..3441029, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1992 domain-containing protein YhdN Neighboring gene 50S ribosomal subunit protein L17 Neighboring gene 30S ribosomal subunit protein S4 Neighboring gene 30S ribosomal subunit protein S11

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure of open promoter complexes with Escherichia coli RNA polymerase as revealed by the DNase I footprinting technique: compilation analysis. Ozoline ON, et al. Nucleic Acids Res, 1995 Nov 25. PMID 8524639, Free PMC Article
    2. The C-terminal domain of the Escherichia coli RNA polymerase alpha subunit plays a role in the CI-dependent activation of the bacteriophage lambda pM promoter. Kedzierska B, et al. Nucleic Acids Res, 2007. PMID 17389649, Free PMC Article
    3. Upstream promoter sequences and alphaCTD mediate stable DNA wrapping within the RNA polymerase-promoter open complex. Cellai S, et al. EMBO Rep, 2007 Mar. PMID 17290289, Free PMC Article
    4. Similarity and divergence between the RNA polymerase alpha subunits from hyperthermophilic Thermotoga maritima and mesophilic Escherichia coli bacteria. Braun F, et al. Gene, 2006 Oct 1. PMID 16859838
    5. Sequence-independent upstream DNA-alphaCTD interactions strongly stimulate Escherichia coli RNA polymerase-lacUV5 promoter association. Ross W, et al. Proc Natl Acad Sci U S A, 2005 Jan 11. PMID 15626760, Free PMC Article

    See all (270) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mutations in AcrR and RNA Polymerase Confer High-Level Resistance to Psoralen-UVA Irradiation.
      Title: Mutations in AcrR and RNA Polymerase Confer High-Level Resistance to Psoralen-UVA Irradiation.
    2. The RNA Polymerase alpha Subunit Recognizes the DNA Shape of the Upstream Promoter Element.
      Title: The RNA Polymerase α Subunit Recognizes the DNA Shape of the Upstream Promoter Element.
    3. Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase has been described.
      Title: Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase.
    4. Overexpression of recombinant inhibitor of host bacterial RNA polymerase (Gp2) in exponentially growing E. coli induces changes in RNAP behavior at single molecule level and induces global alterations in the transcriptional programme.
      Title: Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.
    5. Regulatory interplay between RNA polymerase and nascent RNA structure has been described in detail. (Review)
      Title: A Two-Way Street: Regulatory Interplay between RNA Polymerase and Nascent RNA Structure.
    6. The results suggest that interaction between NusA and NusG could play various regulatory roles during transcription, including recruitment of NusG to RNAP, resynchronization of transcription: translation coupling, and modulation of termination efficiency.
      Title: Transcription is regulated by NusA:NusG interaction.
    7. Our investigations provide molecular insights for understanding how EctopoI interacts with RNAP, a critical step for preventing hypernegative DNA supercoiling during transcription
      Title: Characterization of molecular interactions between Escherichia coli RNA polymerase and topoisomerase I by molecular simulations.
    8. Ribonucleoprotein particles of bacterial small non-coding RNA IsrA and its interaction with RNA polymerase core may link transcription to mRNA fate.
      Title: Ribonucleoprotein particles of bacterial small non-coding RNA IsrA (IS61 or McaS) and its interaction with RNA polymerase core may link transcription to mRNA fate.
    9. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator have been described.
      Title: Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.
    10. These results support the conclusion that bacteriophage Mu Mor recruits RNA polymerase to the bacteriophage middle promoter P(m) rather than reposition a prebound RNA polymerase.
      Title: Unusual interaction of RNA polymerase with the bacteriophage Mu middle promoter Pm in the absence of its activator protein Mor.
    Submit: New GeneRIF Correction See all GeneRIFs (19)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5'-3' RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed 5'-3' RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase I activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase II activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase III activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase IV activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase V activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables plastid single subunit type RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein dimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA-templated transcription initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA-templated transcription initiation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in bacterial-type flagellum assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in bacterial-type flagellum-dependent cell motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cellular response to cell envelope stress NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular iron ion homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in nitrate assimilation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in plastid transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription elongation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription initiation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in response to heat NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in submerged biofilm formation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in transcription antitermination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transcription by RNA polymerase I IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase III IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase IV IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase V IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of DNA-directed RNA polymerase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm HDA PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic DNA-directed RNA polymerase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of cytosolic DNA-directed RNA polymerase complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    RNA polymerase subunit alpha
    NP_417754.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3440040..3441029 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417754.1 RNA polymerase subunit alpha [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417754.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AW69
      Conserved Domains (1) summary
      PRK05182
      Location:6 → 316
      PRK05182; DNA-directed RNA polymerase subunit alpha; Provisional
    Nucleotide Protein
    Heading Accession and Version

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