glpK glycerol kinase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: glpK
Official Full Name: glycerol kinase
Primary source: ECOCYC:EG10398
Locus tag: b3926
See related: ASAP:ABE-0012825
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3918
Summary: The enzyme can undergo a reversible subunit dissociation between tetramer and dimer . [More information is available at EcoCyc: EG10398].
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Genomic context

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Sequence:: NC_000913.3 (4115714..4117222, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

To further elucidate the contributions of the enzymatic (phosphorylation) and alternative (ASTP) functions of GK in adipogenesis, we performed experiments on mammalian GK and E. coli GK. We determined that the ASTP function of GK (which is absent in E. coli GK) plays a greater role than the enzymatic activity in these processes.
Title: The ATP-stimulated translocation promoter (ASTP) activity of glycerol kinase plays central role in adipogenesis.
point mutation promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism
Title: Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol.
Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli.
Title: Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli.
When the functional dimer for glycerol kinase is identified, it is determined that the domain-swap loop is not removed from the catalytic cleft upon dimer formation.
Title: Oligomeric interactions provide alternatives to direct steric modes of control of sugar kinase/actin/hsp70 superfamily functions by heterotropic allosteric effectors: inhibition of E. coli glycerol kinase.
N-terminus verified by Edman degradation on mature peptide
Title: Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme.
GKG230D displays increased enzymatic activity & decreased allosteric regulation by the glycolytic pathway intermediate fructose 1,6-bisphosphate (FBP) compared to wild-type GK.
Title: Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 --> Asp obtained using microfluidic crystallization devices.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables glycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerol catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycerol catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycerol catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycerol metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycerol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerol-3-phosphate metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: glycerol kinase

NP_418361.1

EC 2.7.1.30

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4115714..4117222 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418361.1 glycerol kinase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418361.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9ANG6

Conserved Domains (1) summary

PRK00047
Location:1 → 498

glpK; glycerol kinase GlpK