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    rob DNA-binding transcriptional dual regulator Rob [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948916, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rob
    Official Full Name
    DNA-binding transcriptional dual regulator Rob
    Primary source
    ECOCYC:EG11366
    Locus tag
    b4396
    See related
    ASAP:ABE-0014420
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cbpB; ECK4388; robA
    Summary
    Rob binds to right border of the oriC origin of chromsome replication. [More information is available at EcoGene: EG11366]. Rob is a protein that belongs to the AraC/XylS family. [More information is available at EcoCyc: EG11366].
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    Genomic context

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    Sequence:
    NC_000913.3 (4634441..4635310, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor TrpR Neighboring gene inosine/xanthosine triphosphatase Neighboring gene putative phosphatase Neighboring gene PF05981 family protein CreA Neighboring gene DNA-binding transcriptional regulator CreB Neighboring gene sensory histidine kinase CreC

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Effects of Nucleobase Amino Acids on the Binding of Rob to Its Promoter DNA: Differential Alteration of DNA Affinity and Phenotype. Zhang C, et al. Biochemistry, 2020 Jun 9. PMID 32412234, Free PMC Article
    2. Alteration of Transcriptional Regulator Rob In Vivo: Enhancement of Promoter DNA Binding and Antibiotic Resistance in the Presence of Nucleobase Amino Acids. Zhang C, et al. Biochemistry, 2020 Mar 31. PMID 32157864, Free PMC Article
    3. Structural basis of transcription activation by Rob, a pleiotropic AraC/XylS family regulator. Shi J, et al. Nucleic Acids Res, 2022 Jun 10. PMID 35641097, Free PMC Article
    4. An Escherichia coli curved DNA-binding protein whose expression is affected by the stationary phase-specific sigma factor sigma S. Kakeda M, et al. Mol Gen Genet, 1995 Sep 20. PMID 7476863
    5. The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition. Corbella M, et al. J Phys Chem B, 2021 Jul 1. PMID 34137249, Free PMC Article

    See all (64) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural basis of transcription activation by Rob, a pleiotropic AraC/XylS family regulator.
      Title: Structural basis of transcription activation by Rob, a pleiotropic AraC/XylS family regulator.
    2. The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
      Title: The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
    3. Effects of Nucleobase Amino Acids on the Binding of Rob to Its Promoter DNA: Differential Alteration of DNA Affinity and Phenotype.
      Title: Effects of Nucleobase Amino Acids on the Binding of Rob to Its Promoter DNA: Differential Alteration of DNA Affinity and Phenotype.
    4. Study employs the X-ray crystal structure of transcriptional regulator Rob bound to its micF promoter. Rob proteins modified in vivo with nucleobase amino acids at position 40 exhibited altered DNA promoter binding with promoter DNA A-box residue Gua-6. Rob protein expression ultimately controls phenotypic changes, including resistance to antibiotics.
      Title: Alteration of Transcriptional Regulator Rob In Vivo: Enhancement of Promoter DNA Binding and Antibiotic Resistance in the Presence of Nucleobase Amino Acids.
    5. We speculate that replication pausing and fork-slow-down shortly after initiation may represent a novel checkpoint that ensures the presence of sufficient nucleotide supply prior to commitment to duplication of the entire genome.
      Title: Fork restart protein, PriA, binds around oriC after depletion of nucleotide precursors: Replication fork arrest near the replication origin.
    6. study found that although Rob makes fewer interactions with sigma(70) R4 than SoxS, the 2 proteins make the same position-dependent interactions; Rob occludes sigma(70) R4 from binding the -35 hexamer, just as does SoxS; thus, the C-terminal domain does not substantially alter the way Rob interacts with sigma(70) R4 at class II promoters
      Title: Transcription activation by Escherichia coli Rob at class II promoters: protein-protein interactions between Rob's N-terminal domain and the σ(70) subunit of RNA polymerase.
    7. roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to cationic antimicrobial peptides
      Title: Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon.
    8. Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites.
      Title: An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants.
    9. The authors propose that sequestration inactivates Rob by blocking its access to the transcriptional machinery and that inducers activate Rob by mediating its dispersal, allowing interaction with RNA polymerase.
      Title: Two functions of the C-terminal domain of Escherichia coli Rob: mediating "sequestration-dispersal" as a novel off-on switch for regulating Rob's activity as a transcription activator and preventing degradation of Rob by Lon protease.
    10. The authors show here that tolC has two additional strong overlapping promoters, p3 and p4, which are downstream of p1, p2 and the marbox and are activated by MarA, SoxS and Rob.
      Title: Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables bacterial-type RNA polymerase holo enzyme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator Rob

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4634441..4635310 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418813.1 DNA-binding transcriptional dual regulator Rob [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418813.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A6TXV0, A0A8S7EJM1, A0A8T5ZCK8
      Conserved Domains (1) summary
      PRK15121
      Location:1 → 289
      PRK15121; MDR efflux pump AcrAB transcriptional activator RobA
    Nucleotide Protein
    Heading Accession and Version

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