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    insB1 IS1 family protein InsB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944743, updated on 3-Dec-2024

    Summary

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    Official Symbol
    insB1
    Official Full Name
    IS1 family protein InsB
    Primary source
    ECOCYC:G6085
    Locus tag
    b0021
    See related
    ASAP:ABE-0000075
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0022
    Summary
    IS1 is inserted in 7 locations in MG1655, designated IS1ABCDEFH; IS1G is specific to the W3110 K-12 chromosome. [More information is available at EcoGene: EG60002]. IS1 is the smallest insertion sequence in Escherichia coli. [More information is available at EcoCyc: G6085].
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    Genomic context

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    Sequence:
    NC_000913.3 (19811..20314, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene Na(+):H(+) antiporter NhaA Neighboring gene DNA-binding transcriptional activator NhaR Neighboring gene IS1 family protein InsA Neighboring gene 30S ribosomal subunit protein S20 Neighboring gene DUF2575 domain-containing protein YaaY

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Nucleotide sequence of an insertion element, IS1. Ohtsubo H, et al. Proc Natl Acad Sci U S A, 1978 Feb. PMID 273224, Free PMC Article
    2. Genetic analyses of the interactions of the IS1-encoded proteins with the left end of IS1 and its insertion hotspot. Matsutani S. J Mol Biol, 1997 Apr 4. PMID 9126837
    3. The insE open reading frame of IS1 is not required for formation of cointegrates. Freund ET, et al. J Bacteriol, 1996 Apr. PMID 8636048, Free PMC Article
    4. Frameshifting is required for production of the transposase encoded by insertion sequence 1. Sekine Y, et al. Proc Natl Acad Sci U S A, 1989 Jun. PMID 2543983, Free PMC Article
    5. Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition? Escoubas JM, et al. J Bacteriol, 1994 Sep. PMID 8083181, Free PMC Article

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transposase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA transposition IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transposition IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    IS1 family protein InsB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      19811..20314 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414562.1 IS1 family protein InsB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414562.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0CF31
      UniProtKB/TrEMBL
      A0A0A8J546, A0A2H4TP98
      Conserved Domains (1) summary
      cl00721
      Location:37 → 167
      DDE_Tnp_IS1; IS1 transposase
    Nucleotide Protein
    Heading Accession and Version

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