carB carbamoyl-phosphate synthetase large subunit [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: carB
Official Full Name: carbamoyl-phosphate synthetase large subunit
Primary source: ECOCYC:EG10135
Locus tag: b0033
See related: ASAP:ABE-0000121
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0034; pyrA
Summary: The large subunit is the synthetase component of the enzyme. [More information is available at EcoCyc: EG10135].
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Genomic context

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Sequence:: NC_000913.3 (30817..34038)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results indicate that transcription start site sequence and spacing between the -10 region and the start site are important determinants in establishing the extent of reiterative transcription and levels of productive transcription.
Title: Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.
N-terminus verified by Edman degradation on mature peptide
Title: The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables amino acid binding	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (ammonia) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (ammonia) activity	IEA Inferred from Electronic Annotation more info
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IGI Inferred from Genetic Interaction more info	PubMed
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleotide binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in L-arginine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in L-arginine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in L-arginine biosynthetic process	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within amino acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within pyrimidine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pyrimidine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pyrimidine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of carbamoyl-phosphate synthase complex	IDA Inferred from Direct Assay more info	PubMed
part_of carbamoyl-phosphate synthase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: carbamoyl-phosphate synthetase large subunit

NP_414574.1

EC 6.3.5.5

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

30817..34038

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414574.1 carbamoyl-phosphate synthetase large subunit [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414574.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A2H4TQT1, A0A793CIE4

Conserved Domains (1) summary

PRK05294
Location:1 → 1069

carB; carbamoyl-phosphate synthase large subunit