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    btuF vitamin B12 ABC transporter periplasmic binding protein [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947574, updated on 3-Dec-2024

    Summary

    Official Symbol
    btuF
    Official Full Name
    vitamin B12 ABC transporter periplasmic binding protein
    Primary source
    ECOCYC:EG12334
    Locus tag
    b0158
    See related
    ASAP:ABE-0000542
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0157; yadT
    Summary
    Uncleaved precursor N-terminus was also sequenced, confirming this start site of translation. about 80% of the precursors had the initial Met cleaved. [More information is available at EcoGene: EG12334]. BtuF is the periplasmic vitamin B12 binding protein that delivers cobalamin to the ABC transporter BtuCD. [More information is available at EcoCyc: EG12334].
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    Genomic context

    Sequence:
    NC_000913.3 (177662..178462, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YadW Neighboring gene iron-sulfur cluster insertion protein ErpA Neighboring gene PF03458 family protein YadS Neighboring gene 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Neighboring gene dGTP triphosphohydrolase Neighboring gene periplasmic serine endoprotease DegP

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables cobalamin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables cobalamin binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cobalamin transport IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in cobalamin transport IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in cobalamin transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within cobalamin transport IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    Component Evidence Code Pubs
    part_of cobalamin transport complex IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in periplasmic space IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    vitamin B12 ABC transporter periplasmic binding protein
    NP_414700.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      177662..178462 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414700.1 vitamin B12 ABC transporter periplasmic binding protein [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414700.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FPU6
      Conserved Domains (1) summary
      PRK03379
      Location:7266
      PRK03379; vitamin B12-transporter protein BtuF; Provisional