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    moaD molybdopterin synthase sulfur carrier subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945398, updated on 3-Dec-2024

    Summary

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    Official Symbol
    moaD
    Official Full Name
    molybdopterin synthase sulfur carrier subunit
    Primary source
    ECOCYC:EG11597
    Locus tag
    b0784
    See related
    ASAP:ABE-0002675
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    chlA4; chlM; ECK0773
    Summary
    The C-terminus of MoaD is thiocarboxylated. [More information is available at EcoGene: EG11597]. Molybdopterin synthase catalyzes the second segment of the pathway leading to the synthesis of the molybdenum cofactor, molybdopterin guanine dinucleotide, which chelates molybdate and is present in and essential for the activity of all Escherichia coli's molybdenum-containing enzymes. [More information is available at EcoCyc: EG11597].
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    Genomic context

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    Sequence:
    NC_000913.3 (819048..819293)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene GTP 3',8'-cyclase Neighboring gene protein MoaB Neighboring gene cyclic pyranopterin monophosphate synthase Neighboring gene molybdopterin synthase catalytic subunit Neighboring gene Bax1-I family protein YbhL

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of a molybdopterin synthase-precursor Z complex: insight into its sulfur transfer mechanism and its role in molybdenum cofactor deficiency. Daniels JN, et al. Biochemistry, 2008 Jan 15. PMID 18092812
    2. Reconstitution of plant nitrate reductase by Escherichia coli extracts and the molecular cloning of the chlA gene of Escherichia coli K12. Taylor JL, et al. J Mol Appl Genet, 1983. PMID 6363588
    3. Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin activation. Rudolph MJ, et al. Nat Struct Biol, 2001 Jan. PMID 11135669
    4. Thermodynamic analysis of subunit interactions in Escherichia coli molybdopterin synthase. Tong Y, et al. Biochemistry, 2005 Feb 22. PMID 15709772
    5. Structural studies of molybdopterin synthase provide insights into its catalytic mechanism. Rudolph MJ, et al. J Biol Chem, 2003 Apr 18. PMID 12571227

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MoeB and MoaD can stimulate the IscS activity up to 1.6-fold
      Title: IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli.
    2. N-terminus verified by Edman degradation on complete protein
      Title: The biosynthesis of molybdopterin in Escherichia coli. Purification and characterization of the converting factor.
    3. terminal MoaD residue (Gly81) is important for transfer of sulfur to precursor Z and essential for formation of the MoaD-AMP complex.
      Title: Role of the C-terminal Gly-Gly motif of Escherichia coli MoaD, a molybdenum cofactor biosynthesis protein with a ubiquitin fold.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Mo-molybdopterin cofactor biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in Mo-molybdopterin cofactor biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in Mo-molybdopterin cofactor biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within Mo-molybdopterin cofactor biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of molybdopterin adenylyltransferase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of molybdopterin adenylyltransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of molybdopterin synthase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    molybdopterin synthase sulfur carrier subunit
    NP_415305.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      819048..819293
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415305.1 molybdopterin synthase sulfur carrier subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415305.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5ZF64, A0AAN3KSE4
      Conserved Domains (1) summary
      PRK11130
      Location:1 → 81
      moaD; molybdopterin synthase small subunit; Provisional
    Nucleotide Protein
    Heading Accession and Version

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