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    infC translation initiation factor IF-3 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946225, updated on 3-Dec-2024

    Summary

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    Official Symbol
    infC
    Official Full Name
    translation initiation factor IF-3
    Primary source
    ECOCYC:EG10506
    Locus tag
    b1718
    See related
    ASAP:ABE-0005734
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1716; fit; srjA
    Summary
    HT_Cmplx17_Mem: [More information is available at EcoGene: EG10506]. IF-3 is one of three translation initiation factors in Escherichia coli . [More information is available at EcoCyc: EG10506].
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    Genomic context

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    Sequence:
    NC_000913.3 (1800096..1800638, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L20 Neighboring gene 50S ribosomal subunit protein L35 Neighboring gene threonine--tRNA ligase Neighboring gene pseudo

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Expression of the gene for Escherichia coli initiation factor IE-3 in vivo and in vitro. Lestienne P, et al. Eur J Biochem, 1982 Apr. PMID 7042344
    2. Mapping the active sites of bacterial translation initiation factor IF3. Petrelli D, et al. J Mol Biol, 2003 Aug 15. PMID 12899827
    3. Translation initiation factor IF3: two domains, five functions, one mechanism? Petrelli D, et al. EMBO J, 2001 Aug 15. PMID 11500382, Free PMC Article
    4. Altered discrimination of start codons and initiator tRNAs by mutant initiation factor 3. O'Connor M, et al. RNA, 2001 Jul. PMID 11453069, Free PMC Article
    5. Discrimination by Escherichia coli initiation factor IF3 against initiation on non-canonical codons relies on complementarity rules. Meinnel T, et al. J Mol Biol, 1999 Jul 23. PMID 10398584

    See all (88) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli.
      Title: The initiation factor 3 (IF3) residues interacting with initiator tRNA elbow modulate the fidelity of translation initiation and growth fitness in Escherichia coli.
    2. The premature ribosomes harbouring the assembly defects subvert the IF3 mediated proofreading of cognate initiation codon to enter the translation cycle.
      Title: Ribosome assembly defects subvert initiation Factor3 mediated scrutiny of bona fide start signal.
    3. By gating the transition from 30S ribosome biogenesis to translation initiation, RbfA and IF3 maintain the fidelity of bacterial protein synthesis.
      Title: RbfA and IF3 couple ribosome biogenesis and translation initiation to increase stress tolerance.
    4. The C-terminal domain of infC is essential for growth and the N-terminal domain adds to the fidelity of initiation of protein synthesis and to the fitness of the bacterial growth.
      Title: Contributions of the N- and C-Terminal Domains of Initiation Factor 3 to Its Functions in the Fidelity of Initiation and Antiassociation of the Ribosomal Subunits.
    5. Data show that bacterial initiation factor IF2 accelerated ribosome subunit joining, whereas bacterial initiation factors IF1 and IF3 significantly slowed down subunit association in the absence of IF2.
      Title: Initiation factor 2 stabilizes the ribosome in a semirotated conformation.
    6. These results strongly support a conformational selection model in which the conformation of IF3 that is selectively stabilized within a completely and correctly assembled 30S IC facilitates further progress along the initiation pathway.
      Title: Conformational selection of translation initiation factor 3 signals proper substrate selection.
    7. Ribosomes change into forms which are hard to bind with IF3 and easy to form 100S ribosomes by RMF and HPF concomitantly with transition from the log phase to the stationary phase.
      Title: Activities of Escherichia coli ribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase.
    8. overexpression of IF3 led to a marked decrease in re-initiation efficiency, suggesting that a 30S ribosome and not a 70S ribosome is used for translation re-initiation
      Title: Requirements for translation re-initiation in Escherichia coli: roles of initiator tRNA and initiation factors IF2 and IF3.
    9. N-terminus verified by Edman degradation on complete protein
      Title: The primary structure of the initiation factor IF-3 from Escherichia coli.
    10. Study shows that after cold shock two promoters are activated resulting in de novo infC transcription and IF3 synthesis resulting in IF3/ribosomes ratio that contributes to establishing the cold-shock translational bias.
      Title: Cold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ribosome binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation initiation factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within response to cold IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ribosome disassembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within ribosome disassembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    translation initiation factor IF-3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1800096..1800638 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416233.1 translation initiation factor IF-3 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416233.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AIA3
      Conserved Domains (1) summary
      PRK00028
      Location:5 → 179
      infC; translation initiation factor IF-3; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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    Other Literature Sources
    Molecular Biology Databases
    Research Materials