U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    lepA 30S ribosomal subunit biogenesis factor LepA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947051, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    lepA
    Official Full Name
    30S ribosomal subunit biogenesis factor LepA
    Primary source
    ECOCYC:EG10529
    Locus tag
    b2569
    See related
    ASAP:ABE-0008452
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2567
    Summary
    Although EF4 improves translational accuracy, it can not reverse paromomycin-induced errors (Qin, 2006). [More information is available at EcoGene: EG10529]. LepA is an elongation factor that induces back-translocation of the ribosome. [More information is available at EcoCyc: EG10529].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2705325..2707124, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit maturation GTPase Era Neighboring gene RNase III Neighboring gene signal peptidase I Neighboring gene protein RseC Neighboring gene anti-sigma factor stabilizing protein RseB Neighboring gene anti-sigma-E factor RseA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Interrupted catalysis: the EF4 (LepA) effect on back-translocation. Liu H, et al. J Mol Biol, 2010 Mar 5. PMID 20045415, Free PMC Article
    2. Small clusters of divergent amino acids surrounding the effector domain mediate the varied phenotypes of EF-G and LepA expression. Yaskowiak ES, et al. Mol Microbiol, 1995 Mar. PMID 7596295
    3. The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. Qin Y, et al. Cell, 2006 Nov 17. PMID 17110332
    4. Ribosomal translocation: LepA does it backwards. Youngman EM, et al. Curr Biol, 2007 Feb 20. PMID 17307049
    5. The structure of LepA, the ribosomal back translocase. Evans RN, et al. Proc Natl Acad Sci U S A, 2008 Mar 25. PMID 18362332, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The last 44 C-terminal amino acids of EF4 form a sub-domain within the C-terminal domain that is important for GTP-dependent function on the ribosome.
      Title: Identification of two structural elements important for ribosome-dependent GTPase activity of elongation factor 4 (EF4/LepA).
    2. Thus, EF4 has two opposing functions-at low-to-moderate levels of stress, the protein is protective by allowing stress-paused translation to resume; at high-levels of stress, EF4 helps bacteria self-destruct.
      Title: Ribosomal elongation factor 4 promotes cell death associated with lethal stress.
    3. (review) EF4 triggers back-translocation of the elongating ribosome, causing the translation machinery to move one codon backwards along the mRNA.
      Title: The paradox of elongation factor 4: highly conserved, yet of no physiological significance?
    4. Elevated intracellular Mg(2+) concentrations or low temperature inhibit protein synthesis, probably because of formation of aberrant elongating ribosomal states. We suggest that EF4 binds to these stuck ribosomes and remobilizes them
      Title: Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations.
    5. EF4-dependent back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex via a four-step kinetic scheme (i.e., POST-->I(1)-->I(2)-->I(3)-->PRE
      Title: Interrupted catalysis: the EF4 (LepA) effect on back-translocation.
    6. The crystal structure of LepA from Escherichia coli at 2.8-A resolution, was determined.
      Title: The structure of LepA, the ribosomal back translocase.
    7. LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis.
      Title: The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanosine tetraphosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables membrane scission GTPase motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ribosomal large subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosomal small subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosome binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ribosome binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables translation elongation factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of translation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to cold IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to pH IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to salt stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within ribosomal small subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational elongation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    30S ribosomal subunit biogenesis factor LepA
    NP_417064.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2705325..2707124 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417064.1 30S ribosomal subunit biogenesis factor LepA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417064.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1F6E5
      Conserved Domains (1) summary
      PRK05433
      Location:1 → 597
      PRK05433; GTP-binding protein LepA; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.