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    ispF 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945057, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ispF
    Official Full Name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Primary source
    ECOCYC:EG11816
    Locus tag
    b2746
    See related
    ASAP:ABE-0009013
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2741; ygbB
    Summary
    2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF) catalyzes the fifth step in the methylerythritol phosphate pathway. [More information is available at EcoCyc: EG11816].
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    Genomic context

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    Sequence:
    NC_000913.3 (2871301..2871780, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene murein hydrolase activator NlpD Neighboring gene protein-L-isoaspartate O-methyltransferase Neighboring gene broad specificity 5'(3')-nucleotidase and polyphosphatase Neighboring gene tRNA pseudouridine(13) synthase Neighboring gene 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase Neighboring gene cell division protein FtsB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A structure-based approach to ligand discovery for 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase: a target for antimicrobial therapy. Ramsden NL, et al. J Med Chem, 2009 Apr 23. PMID 19320487, Free PMC Article
    2. A double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate. Sgraja T, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2005 Jul 1. PMID 16511114, Free PMC Article
    3. The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods. Kemp LE, et al. Acta Crystallogr D Biol Crystallogr, 2005 Jan. PMID 15608374
    4. Characterization of the depletion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase in Escherichia coli and Bacillus subtilis. Campbell TL, et al. J Bacteriol, 2002 Oct. PMID 12270818, Free PMC Article
    5. Structure of 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase: an essential enzyme for isoprenoid biosynthesis and target for antimicrobial drug development. Kemp LE, et al. Proc Natl Acad Sci U S A, 2002 May 14. PMID 11997478, Free PMC Article

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. the double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate
      Title: A double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate.
    2. MECP synthase binds three metabolites that are produced by enzymes two, three and four stages downstream in isoprenoid biosynthesis
      Title: The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in isoprenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in terpenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within ubiquinone biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    NP_417226.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2871301..2871780 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417226.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417226.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MH63
      Conserved Domains (1) summary
      PRK00084
      Location:1 → 157
      ispF; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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