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    dam DNA adenine methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947893, updated on 17-Dec-2024

    Summary

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    Official Symbol
    dam
    Official Full Name
    DNA adenine methyltransferase
    Primary source
    ECOCYC:EG10204
    Locus tag
    b3387
    See related
    ASAP:ABE-0011063
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3374
    Summary
    Dam is a base flipping enzyme. [More information is available at EcoGene: EG10204]. DNA adenine methyltransferase (Dam), which is responsible for methylation of nearly all GATC sequences in Escherichia coli , is encoded by the dam gene and occurs at about 130 molecules per rapidly growing cell . [More information is available at EcoCyc: EG10204].
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    Genomic context

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    Sequence:
    NC_000913.3 (3515077..3515913, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene tryptophan--tRNA ligase Neighboring gene phosphoglycolate phosphatase Neighboring gene ribulose-phosphate 3-epimerase Neighboring gene cell division protein DamX Neighboring gene 3-dehydroquinate synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12. McGraw BR, et al. Mol Gen Genet, 1980. PMID 6993844
    2. N6-methyl-adenine: an epigenetic signal for DNA-protein interactions. Wion D, et al. Nat Rev Microbiol, 2006 Mar. PMID 16489347, Free PMC Article
    3. Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA. Matic I, et al. J Bacteriol, 2006 Jan. PMID 16352851, Free PMC Article
    4. GATC sequence and mismatch repair in Escherichia coli. Laengle-Rouault F, et al. EMBO J, 1986 Aug. PMID 3019677, Free PMC Article
    5. Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli. Smith DW, et al. EMBO J, 1985 May. PMID 3891329, Free PMC Article

    See all (94) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DNA adenine methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli.
      Title: DNA adenine methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli.
    2. Fur-Dam Regulatory Interplay at an Internal Promoter of the Enteroaggregative Escherichia coli Type VI Secretion sci1 Gene Cluster.
      Title: Fur-Dam Regulatory Interplay at an Internal Promoter of the Enteroaggregative Escherichia coli Type VI Secretion sci1 Gene Cluster.
    3. Structural analysis of a new Dam binding site on DNA with implications for methylation-independent transcriptional repression.
      Title: Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.
    4. We propose a model for intrasite methylation in which Dam bound to flanking DNA is an obligate intermediate.
      Title: Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: mechanistic exploration of epigenetic gene regulation.
    5. Unlike other restriction-modification methyltransferases, E. coli DNA adenine methyltransferase carries out intrasite processive catalysis whereby the enzyme-DNA complex methylates both strands of unmethylated GATC site prior to dissociation from DNA.
      Title: Escherichia coli DNA adenine methyltransferase: intrasite processivity and substrate-induced dimerization and activation.
    6. only at low Lrp concentrations will Dam compete effectively for binding and methylation of the proximal GATC site, leading to a phase switch resulting in the expression of pili
      Title: Competitive Lrp and Dam assembly at the pap regulatory region: implications for mechanisms of epigenetic regulation.
    7. Lys(14) is important for EcoDam binding of the extrahelical target base into the active site pocket
      Title: Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle.
    8. structural and functional analysis
      Title: Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics.
    9. the up-regulation of recombinational repair in dam mutants allows for the efficient repair of double-strand breaks whose formation is dependent on functional mismatch repair
      Title: Analysis of global gene expression and double-strand-break formation in DNA adenine methyltransferase- and mismatch repair-deficient Escherichia coli.
    10. DNA regions accessible to Dam methylase in transfected human cells apparently coincided with those hypersensitive to DNase I.
      Title: Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E. coli Dam methylase.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables S-adenosyl-L-methionine binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables S-adenosyl-L-methionine binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables site-specific DNA-methyltransferase (adenine-specific) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables site-specific DNA-methyltransferase (adenine-specific) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables site-specific DNA-methyltransferase (adenine-specific) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA restriction-modification system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA-templated DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within bacterial-type DNA replication initiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within mismatch repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in mismatch repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within mismatch repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within mismatch repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to UV IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA adenine methyltransferase
    NP_417846.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3515077..3515913 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417846.1 DNA adenine methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417846.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1EVR9
      Conserved Domains (1) summary
      PRK10904
      Location:1 → 271
      PRK10904; adenine-specific DNA-methyltransferase
    Nucleotide Protein
    Heading Accession and Version

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